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Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing

A series of wound membranes of polyvinyl alcohol and recombinant spider silk protein (pNSR16) was prepared by electrospinning. The membrane was analyzed by scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy. The result showed that the three factors tha...

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Detalles Bibliográficos
Autores principales: Zhao, Liang, Chen, Denglong, Yao, Qinghua, Li, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679674/
https://www.ncbi.nlm.nih.gov/pubmed/29138566
http://dx.doi.org/10.2147/IJN.S47256
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author Zhao, Liang
Chen, Denglong
Yao, Qinghua
Li, Min
author_facet Zhao, Liang
Chen, Denglong
Yao, Qinghua
Li, Min
author_sort Zhao, Liang
collection PubMed
description A series of wound membranes of polyvinyl alcohol and recombinant spider silk protein (pNSR16) was prepared by electrospinning. The membrane was analyzed by scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy. The result showed that the three factors that affected average fiber diameter from high to low were, voltage, flow speed, and solidification distance; and the three factors that affected fiber uniformity from high to low were, flow speed, solidification distance, and voltage. The fibers adhered together after being dealt with alcohol. pNSR16 transformed from random coil into β-sheet after being immersed in alcohol. Additionally, the porosity of the electrospun membrane was 84.85%, which was higher than that of cast membrane prepared with the same composition. Experiments of applying electrospun membranes as wound dressing for Sprague Dawley rat wound healing showed that it could promote wound healing and basic fibroblast growth factor expression.
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spelling pubmed-56796742017-11-14 Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing Zhao, Liang Chen, Denglong Yao, Qinghua Li, Min Int J Nanomedicine Original Research A series of wound membranes of polyvinyl alcohol and recombinant spider silk protein (pNSR16) was prepared by electrospinning. The membrane was analyzed by scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy. The result showed that the three factors that affected average fiber diameter from high to low were, voltage, flow speed, and solidification distance; and the three factors that affected fiber uniformity from high to low were, flow speed, solidification distance, and voltage. The fibers adhered together after being dealt with alcohol. pNSR16 transformed from random coil into β-sheet after being immersed in alcohol. Additionally, the porosity of the electrospun membrane was 84.85%, which was higher than that of cast membrane prepared with the same composition. Experiments of applying electrospun membranes as wound dressing for Sprague Dawley rat wound healing showed that it could promote wound healing and basic fibroblast growth factor expression. Dove Medical Press 2017-11-02 /pmc/articles/PMC5679674/ /pubmed/29138566 http://dx.doi.org/10.2147/IJN.S47256 Text en © 2017 Zhao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zhao, Liang
Chen, Denglong
Yao, Qinghua
Li, Min
Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_full Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_fullStr Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_full_unstemmed Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_short Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_sort studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679674/
https://www.ncbi.nlm.nih.gov/pubmed/29138566
http://dx.doi.org/10.2147/IJN.S47256
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