Cargando…

The NOD2 receptor is crucial for immune responses towards New World Leishmania species

American Tegumentary Leishmaniasis is a chronic infection caused by Leishmania protozoan. It is not known whether genetic variances in NOD-like receptor (NLR) family members influence the immune response towards Leishmania parasites and modulate intracellular killing. Using functional genomics, we i...

Descripción completa

Detalles Bibliográficos
Autores principales: dos Santos, Jéssica Cristina, Damen, Michelle S. M. A., Oosting, Marije, de Jong, Dirk J., Heinhuis, Bas, Gomes, Rodrigo Saar, Araújo, Carla Santos, Netea, Mihai G., Ribeiro-Dias, Fátima, Joosten, Leo A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680260/
https://www.ncbi.nlm.nih.gov/pubmed/29123157
http://dx.doi.org/10.1038/s41598-017-15412-7
Descripción
Sumario:American Tegumentary Leishmaniasis is a chronic infection caused by Leishmania protozoan. It is not known whether genetic variances in NOD-like receptor (NLR) family members influence the immune response towards Leishmania parasites and modulate intracellular killing. Using functional genomics, we investigated whether genetic variants in NOD1 or NOD2 influence the production of cytokines by human PBMCs exposed to Leishmania. In addition, we examined whether recognition of Leishmania by NOD2 contributes to intracellular killing. Polymorphisms in the NOD2 gene decreased monocyte- and lymphocyte-derived cytokine production after stimulation with L. amazonensis or L. braziliensis compared to individuals with a functional NOD2 receptor. The phagolysosome formation is important for Leishmania-induced cytokine production and upregulation of NOD2 mRNA expression. NOD2 is crucial to control intracellular infection caused by Leishmania spp. NOD2 receptor is important for Leishmania recognition, the control of intracellular killing, and the induction of innate and adaptive immune responses.