The NOD2 receptor is crucial for immune responses towards New World Leishmania species

American Tegumentary Leishmaniasis is a chronic infection caused by Leishmania protozoan. It is not known whether genetic variances in NOD-like receptor (NLR) family members influence the immune response towards Leishmania parasites and modulate intracellular killing. Using functional genomics, we i...

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Autores principales: dos Santos, Jéssica Cristina, Damen, Michelle S. M. A., Oosting, Marije, de Jong, Dirk J., Heinhuis, Bas, Gomes, Rodrigo Saar, Araújo, Carla Santos, Netea, Mihai G., Ribeiro-Dias, Fátima, Joosten, Leo A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680260/
https://www.ncbi.nlm.nih.gov/pubmed/29123157
http://dx.doi.org/10.1038/s41598-017-15412-7
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author dos Santos, Jéssica Cristina
Damen, Michelle S. M. A.
Oosting, Marije
de Jong, Dirk J.
Heinhuis, Bas
Gomes, Rodrigo Saar
Araújo, Carla Santos
Netea, Mihai G.
Ribeiro-Dias, Fátima
Joosten, Leo A. B.
author_facet dos Santos, Jéssica Cristina
Damen, Michelle S. M. A.
Oosting, Marije
de Jong, Dirk J.
Heinhuis, Bas
Gomes, Rodrigo Saar
Araújo, Carla Santos
Netea, Mihai G.
Ribeiro-Dias, Fátima
Joosten, Leo A. B.
author_sort dos Santos, Jéssica Cristina
collection PubMed
description American Tegumentary Leishmaniasis is a chronic infection caused by Leishmania protozoan. It is not known whether genetic variances in NOD-like receptor (NLR) family members influence the immune response towards Leishmania parasites and modulate intracellular killing. Using functional genomics, we investigated whether genetic variants in NOD1 or NOD2 influence the production of cytokines by human PBMCs exposed to Leishmania. In addition, we examined whether recognition of Leishmania by NOD2 contributes to intracellular killing. Polymorphisms in the NOD2 gene decreased monocyte- and lymphocyte-derived cytokine production after stimulation with L. amazonensis or L. braziliensis compared to individuals with a functional NOD2 receptor. The phagolysosome formation is important for Leishmania-induced cytokine production and upregulation of NOD2 mRNA expression. NOD2 is crucial to control intracellular infection caused by Leishmania spp. NOD2 receptor is important for Leishmania recognition, the control of intracellular killing, and the induction of innate and adaptive immune responses.
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spelling pubmed-56802602017-11-17 The NOD2 receptor is crucial for immune responses towards New World Leishmania species dos Santos, Jéssica Cristina Damen, Michelle S. M. A. Oosting, Marije de Jong, Dirk J. Heinhuis, Bas Gomes, Rodrigo Saar Araújo, Carla Santos Netea, Mihai G. Ribeiro-Dias, Fátima Joosten, Leo A. B. Sci Rep Article American Tegumentary Leishmaniasis is a chronic infection caused by Leishmania protozoan. It is not known whether genetic variances in NOD-like receptor (NLR) family members influence the immune response towards Leishmania parasites and modulate intracellular killing. Using functional genomics, we investigated whether genetic variants in NOD1 or NOD2 influence the production of cytokines by human PBMCs exposed to Leishmania. In addition, we examined whether recognition of Leishmania by NOD2 contributes to intracellular killing. Polymorphisms in the NOD2 gene decreased monocyte- and lymphocyte-derived cytokine production after stimulation with L. amazonensis or L. braziliensis compared to individuals with a functional NOD2 receptor. The phagolysosome formation is important for Leishmania-induced cytokine production and upregulation of NOD2 mRNA expression. NOD2 is crucial to control intracellular infection caused by Leishmania spp. NOD2 receptor is important for Leishmania recognition, the control of intracellular killing, and the induction of innate and adaptive immune responses. Nature Publishing Group UK 2017-11-09 /pmc/articles/PMC5680260/ /pubmed/29123157 http://dx.doi.org/10.1038/s41598-017-15412-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
dos Santos, Jéssica Cristina
Damen, Michelle S. M. A.
Oosting, Marije
de Jong, Dirk J.
Heinhuis, Bas
Gomes, Rodrigo Saar
Araújo, Carla Santos
Netea, Mihai G.
Ribeiro-Dias, Fátima
Joosten, Leo A. B.
The NOD2 receptor is crucial for immune responses towards New World Leishmania species
title The NOD2 receptor is crucial for immune responses towards New World Leishmania species
title_full The NOD2 receptor is crucial for immune responses towards New World Leishmania species
title_fullStr The NOD2 receptor is crucial for immune responses towards New World Leishmania species
title_full_unstemmed The NOD2 receptor is crucial for immune responses towards New World Leishmania species
title_short The NOD2 receptor is crucial for immune responses towards New World Leishmania species
title_sort nod2 receptor is crucial for immune responses towards new world leishmania species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680260/
https://www.ncbi.nlm.nih.gov/pubmed/29123157
http://dx.doi.org/10.1038/s41598-017-15412-7
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