Cargando…
Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer
BACKGROUND: Aldehyde dehydrogenase (ALDH) 1A1 is an immunohistological biomarker of various solid tumours, but has not been successfully proved as a colorectal cancer (CRC) marker. We recently reported that ALDH1B1, which has functional roles in tumourigenesis, may be a better CRC marker than ALDH1A...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680456/ https://www.ncbi.nlm.nih.gov/pubmed/28881356 http://dx.doi.org/10.1038/bjc.2017.304 |
_version_ | 1783277763348660224 |
---|---|
author | Matsumoto, Akiko Arcaroli, John Chen, Ying Gasparetto, Maura Neumeister, Veronique Thompson, David C Singh, Surendra Smith, Clayton Messersmith, Wells Vasiliou, Vasilis |
author_facet | Matsumoto, Akiko Arcaroli, John Chen, Ying Gasparetto, Maura Neumeister, Veronique Thompson, David C Singh, Surendra Smith, Clayton Messersmith, Wells Vasiliou, Vasilis |
author_sort | Matsumoto, Akiko |
collection | PubMed |
description | BACKGROUND: Aldehyde dehydrogenase (ALDH) 1A1 is an immunohistological biomarker of various solid tumours, but has not been successfully proved as a colorectal cancer (CRC) marker. We recently reported that ALDH1B1, which has functional roles in tumourigenesis, may be a better CRC marker than ALDH1A1. METHODS: Human CRC explants and cell lines were analysed to identify candidate CRC markers from eight ALDH isozymes including ALDH1A1 and ALDH1B1. A tissue microarray, including paired specimens of normal and tumour tissues, was subsequently analysed to determine if candidate ALDHs could distinguish CRC from normal tissue. RESULTS: Based on mRNA analysis, ALDH1B1 and ALDH2 were selected as suitable candidates. These were strongly and regularly expressed in tumour tissue and cell lines, including highly tumourigenic cell populations (ALDH(+)CD44(+) cells), while other ALDHs, including ALDH1A1, showed differential or low expression. No genetic alteration of ALDH1B1 in CRC was suggested by the relationships between mRNA and protein levels/enzymatic activities, and cDNA sequences of CRC cell lines. Tissue microarray findings showed that ALDH1B1, but not ALDH2, could distinguish CRC from normal tissue. Furthermore, ratios of ALDH1B1 to ALDH1A1 or ALDH2 were found to be powerful CRC indicators. CONCLUSIONS: These results suggest that ALDH1B1 is a novel human CRC biomarker. |
format | Online Article Text |
id | pubmed-5680456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-56804562018-11-07 Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer Matsumoto, Akiko Arcaroli, John Chen, Ying Gasparetto, Maura Neumeister, Veronique Thompson, David C Singh, Surendra Smith, Clayton Messersmith, Wells Vasiliou, Vasilis Br J Cancer Molecular Diagnostics BACKGROUND: Aldehyde dehydrogenase (ALDH) 1A1 is an immunohistological biomarker of various solid tumours, but has not been successfully proved as a colorectal cancer (CRC) marker. We recently reported that ALDH1B1, which has functional roles in tumourigenesis, may be a better CRC marker than ALDH1A1. METHODS: Human CRC explants and cell lines were analysed to identify candidate CRC markers from eight ALDH isozymes including ALDH1A1 and ALDH1B1. A tissue microarray, including paired specimens of normal and tumour tissues, was subsequently analysed to determine if candidate ALDHs could distinguish CRC from normal tissue. RESULTS: Based on mRNA analysis, ALDH1B1 and ALDH2 were selected as suitable candidates. These were strongly and regularly expressed in tumour tissue and cell lines, including highly tumourigenic cell populations (ALDH(+)CD44(+) cells), while other ALDHs, including ALDH1A1, showed differential or low expression. No genetic alteration of ALDH1B1 in CRC was suggested by the relationships between mRNA and protein levels/enzymatic activities, and cDNA sequences of CRC cell lines. Tissue microarray findings showed that ALDH1B1, but not ALDH2, could distinguish CRC from normal tissue. Furthermore, ratios of ALDH1B1 to ALDH1A1 or ALDH2 were found to be powerful CRC indicators. CONCLUSIONS: These results suggest that ALDH1B1 is a novel human CRC biomarker. Nature Publishing Group 2017-11-07 2017-09-07 /pmc/articles/PMC5680456/ /pubmed/28881356 http://dx.doi.org/10.1038/bjc.2017.304 Text en Copyright © 2017 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/4.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Molecular Diagnostics Matsumoto, Akiko Arcaroli, John Chen, Ying Gasparetto, Maura Neumeister, Veronique Thompson, David C Singh, Surendra Smith, Clayton Messersmith, Wells Vasiliou, Vasilis Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer |
title | Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer |
title_full | Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer |
title_fullStr | Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer |
title_full_unstemmed | Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer |
title_short | Aldehyde dehydrogenase 1B1: a novel immunohistological marker for colorectal cancer |
title_sort | aldehyde dehydrogenase 1b1: a novel immunohistological marker for colorectal cancer |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5680456/ https://www.ncbi.nlm.nih.gov/pubmed/28881356 http://dx.doi.org/10.1038/bjc.2017.304 |
work_keys_str_mv | AT matsumotoakiko aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT arcarolijohn aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT chenying aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT gasparettomaura aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT neumeisterveronique aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT thompsondavidc aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT singhsurendra aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT smithclayton aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT messersmithwells aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer AT vasiliouvasilis aldehydedehydrogenase1b1anovelimmunohistologicalmarkerforcolorectalcancer |