Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy

The CRISPR-associated endonuclease Cas9 binds to a guide RNA and cleaves double-stranded DNA with a sequence complementary to the RNA guide. The Cas9–RNA system has been harnessed for numerous applications, such as genome editing. Here we use high-speed atomic force microscopy (HS-AFM) to visualize...

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Detalles Bibliográficos
Autores principales: Shibata, Mikihiro, Nishimasu, Hiroshi, Kodera, Noriyuki, Hirano, Seiichi, Ando, Toshio, Uchihashi, Takayuki, Nureki, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681550/
https://www.ncbi.nlm.nih.gov/pubmed/29127285
http://dx.doi.org/10.1038/s41467-017-01466-8

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