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Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism

As an important signal molecule, salicylic acid (SA) improves plant tolerance to aluminum (Al) stress. The objective of this study was to investigate the effects of exogenous SA application on the dynamics of endogenous SA and reactive oxygen species in soybean (Glycine max L.) exposed to Al stress....

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Autores principales: Liu, Ning, Song, Fengbin, Zhu, Xiancan, You, Jiangfeng, Yang, Zhenming, Li, Xiangnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681908/
https://www.ncbi.nlm.nih.gov/pubmed/29164108
http://dx.doi.org/10.3389/fchem.2017.00096
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author Liu, Ning
Song, Fengbin
Zhu, Xiancan
You, Jiangfeng
Yang, Zhenming
Li, Xiangnan
author_facet Liu, Ning
Song, Fengbin
Zhu, Xiancan
You, Jiangfeng
Yang, Zhenming
Li, Xiangnan
author_sort Liu, Ning
collection PubMed
description As an important signal molecule, salicylic acid (SA) improves plant tolerance to aluminum (Al) stress. The objective of this study was to investigate the effects of exogenous SA application on the dynamics of endogenous SA and reactive oxygen species in soybean (Glycine max L.) exposed to Al stress. The roots of soybean seedlings were exposed to a combination of AlCl(3) (30 μM) and SA (10 μM)/PAC (100 μM, paclobutrazol, SA biosynthesis inhibitor) for 3, 6, 9, and 12 h. Al stress induced an increase in endogenous SA concentration in a time-dependent manner, also verified by the up-regulated expression of GmNPR1, an SA-responsive gene. Al stress increased the activities of phenylalanine ammonia-lyase (PAL) and benzoic acid 2-hydroxylase (BA2H), and the contents of SA, [Formula: see text] and malondialdehyde (MDA) in the root apex. The application of exogenous SA increased PAL and BA2H, and reduced [Formula: see text] and MDA contents in soybean roots under Al stress. PAC inhibited the SA induced increase in BA2H activity. In addition, the SA application resulted in a rapid increase in hydrogen peroxide (H(2)O(2)) concentration under Al stress, followed by a sharp decrease. Compared with the plants exposed to Al alone, Al+SA plants possessed higher activities of superoxide dismutase, peroxidase, and ascorbate peroxidase, and lower catalase activity, indicating that SA alleviated Al-induced oxidative damage. These results suggested that PAL and BA2H were involved in Al-induced SA production and showed that SA alleviated the adverse effects of Al toxicity by modulating the cellular H(2)O(2) level and the antioxidant enzyme activities in the soybean root apex.
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spelling pubmed-56819082017-11-21 Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism Liu, Ning Song, Fengbin Zhu, Xiancan You, Jiangfeng Yang, Zhenming Li, Xiangnan Front Chem Chemistry As an important signal molecule, salicylic acid (SA) improves plant tolerance to aluminum (Al) stress. The objective of this study was to investigate the effects of exogenous SA application on the dynamics of endogenous SA and reactive oxygen species in soybean (Glycine max L.) exposed to Al stress. The roots of soybean seedlings were exposed to a combination of AlCl(3) (30 μM) and SA (10 μM)/PAC (100 μM, paclobutrazol, SA biosynthesis inhibitor) for 3, 6, 9, and 12 h. Al stress induced an increase in endogenous SA concentration in a time-dependent manner, also verified by the up-regulated expression of GmNPR1, an SA-responsive gene. Al stress increased the activities of phenylalanine ammonia-lyase (PAL) and benzoic acid 2-hydroxylase (BA2H), and the contents of SA, [Formula: see text] and malondialdehyde (MDA) in the root apex. The application of exogenous SA increased PAL and BA2H, and reduced [Formula: see text] and MDA contents in soybean roots under Al stress. PAC inhibited the SA induced increase in BA2H activity. In addition, the SA application resulted in a rapid increase in hydrogen peroxide (H(2)O(2)) concentration under Al stress, followed by a sharp decrease. Compared with the plants exposed to Al alone, Al+SA plants possessed higher activities of superoxide dismutase, peroxidase, and ascorbate peroxidase, and lower catalase activity, indicating that SA alleviated Al-induced oxidative damage. These results suggested that PAL and BA2H were involved in Al-induced SA production and showed that SA alleviated the adverse effects of Al toxicity by modulating the cellular H(2)O(2) level and the antioxidant enzyme activities in the soybean root apex. Frontiers Media S.A. 2017-11-07 /pmc/articles/PMC5681908/ /pubmed/29164108 http://dx.doi.org/10.3389/fchem.2017.00096 Text en Copyright © 2017 Liu, Song, Zhu, You, Yang and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Liu, Ning
Song, Fengbin
Zhu, Xiancan
You, Jiangfeng
Yang, Zhenming
Li, Xiangnan
Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism
title Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism
title_full Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism
title_fullStr Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism
title_full_unstemmed Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism
title_short Salicylic Acid Alleviates Aluminum Toxicity in Soybean Roots through Modulation of Reactive Oxygen Species Metabolism
title_sort salicylic acid alleviates aluminum toxicity in soybean roots through modulation of reactive oxygen species metabolism
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681908/
https://www.ncbi.nlm.nih.gov/pubmed/29164108
http://dx.doi.org/10.3389/fchem.2017.00096
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