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In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer

Fermentation of undigested proteins in human gastrointestinal tract (gut) by the resident microbiota, a process called bacterial putrefaction, can sometimes disrupt the gut homeostasis. In this process, essential amino acids (e.g., histidine, tryptophan, etc.) that are required by the host may be ut...

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Autores principales: Kaur, Harrisham, Das, Chandrani, Mande, Sharmila S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682003/
https://www.ncbi.nlm.nih.gov/pubmed/29163445
http://dx.doi.org/10.3389/fmicb.2017.02166
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author Kaur, Harrisham
Das, Chandrani
Mande, Sharmila S.
author_facet Kaur, Harrisham
Das, Chandrani
Mande, Sharmila S.
author_sort Kaur, Harrisham
collection PubMed
description Fermentation of undigested proteins in human gastrointestinal tract (gut) by the resident microbiota, a process called bacterial putrefaction, can sometimes disrupt the gut homeostasis. In this process, essential amino acids (e.g., histidine, tryptophan, etc.) that are required by the host may be utilized by the gut microbes. In addition, some of the products of putrefaction, like ammonia, putrescine, cresol, indole, phenol, etc., have been implicated in the disease pathogenesis of colorectal cancer (CRC). We have investigated bacterial putrefaction pathways that are known to be associated with such metabolites. Results of the comprehensive in silico analysis of the selected putrefaction pathways across bacterial genomes revealed presence of these pathways in limited bacterial groups. Majority of these bacteria are commonly found in human gut. These include Bacillus, Clostridium, Enterobacter, Escherichia, Fusobacterium, Salmonella, etc. Interestingly, while pathogens utilize almost all the analyzed pathways, commensals prefer putrescine and H(2)S production pathways for metabolizing the undigested proteins. Further, comparison of the putrefaction pathways in the gut microbiomes of healthy, carcinoma and adenoma datasets indicate higher abundances of putrefying bacteria in the carcinoma stage of CRC. The insights obtained from the present study indicate utilization of possible microbiome-based therapies to minimize the adverse effects of gut microbiome in enteric diseases.
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spelling pubmed-56820032017-11-21 In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer Kaur, Harrisham Das, Chandrani Mande, Sharmila S. Front Microbiol Microbiology Fermentation of undigested proteins in human gastrointestinal tract (gut) by the resident microbiota, a process called bacterial putrefaction, can sometimes disrupt the gut homeostasis. In this process, essential amino acids (e.g., histidine, tryptophan, etc.) that are required by the host may be utilized by the gut microbes. In addition, some of the products of putrefaction, like ammonia, putrescine, cresol, indole, phenol, etc., have been implicated in the disease pathogenesis of colorectal cancer (CRC). We have investigated bacterial putrefaction pathways that are known to be associated with such metabolites. Results of the comprehensive in silico analysis of the selected putrefaction pathways across bacterial genomes revealed presence of these pathways in limited bacterial groups. Majority of these bacteria are commonly found in human gut. These include Bacillus, Clostridium, Enterobacter, Escherichia, Fusobacterium, Salmonella, etc. Interestingly, while pathogens utilize almost all the analyzed pathways, commensals prefer putrescine and H(2)S production pathways for metabolizing the undigested proteins. Further, comparison of the putrefaction pathways in the gut microbiomes of healthy, carcinoma and adenoma datasets indicate higher abundances of putrefying bacteria in the carcinoma stage of CRC. The insights obtained from the present study indicate utilization of possible microbiome-based therapies to minimize the adverse effects of gut microbiome in enteric diseases. Frontiers Media S.A. 2017-11-07 /pmc/articles/PMC5682003/ /pubmed/29163445 http://dx.doi.org/10.3389/fmicb.2017.02166 Text en Copyright © 2017 Kaur, Das and Mande. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kaur, Harrisham
Das, Chandrani
Mande, Sharmila S.
In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer
title In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer
title_full In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer
title_fullStr In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer
title_full_unstemmed In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer
title_short In Silico Analysis of Putrefaction Pathways in Bacteria and Its Implication in Colorectal Cancer
title_sort in silico analysis of putrefaction pathways in bacteria and its implication in colorectal cancer
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682003/
https://www.ncbi.nlm.nih.gov/pubmed/29163445
http://dx.doi.org/10.3389/fmicb.2017.02166
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