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Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say

Pest control in the agricultural fields, a major concern globally, is currently achieved through chemical or biological methods. Chemical methods, which leave toxic residue in the produce, are less preferred than biological methods. Venoms injected by stings of various wasps that kill the pest is co...

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Autores principales: Saba, Evelyn, Shafeeq, Tahir, Irfan, Muhammad, Lee, Yuan Yee, Kwon, Hyuk-Woo, Seo, Myung Gi, Park, Sang-Joon, Lee, Kyeong-Yeoll, Rhee, Man Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682083/
https://www.ncbi.nlm.nih.gov/pubmed/29213193
http://dx.doi.org/10.1155/2017/6978194
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author Saba, Evelyn
Shafeeq, Tahir
Irfan, Muhammad
Lee, Yuan Yee
Kwon, Hyuk-Woo
Seo, Myung Gi
Park, Sang-Joon
Lee, Kyeong-Yeoll
Rhee, Man Hee
author_facet Saba, Evelyn
Shafeeq, Tahir
Irfan, Muhammad
Lee, Yuan Yee
Kwon, Hyuk-Woo
Seo, Myung Gi
Park, Sang-Joon
Lee, Kyeong-Yeoll
Rhee, Man Hee
author_sort Saba, Evelyn
collection PubMed
description Pest control in the agricultural fields, a major concern globally, is currently achieved through chemical or biological methods. Chemical methods, which leave toxic residue in the produce, are less preferred than biological methods. Venoms injected by stings of various wasps that kill the pest is considered as the examples of the biological method. Although several studies have investigated the biological control of pests through these venoms, very few studies have reported the effects of these venoms on mammalian cells. Bracon hebetor, an ectoparasitoid of the order Hymenoptera, is having a paramount importance in parasitizing various lepidopterous larvae including Plodia interpunctella also called as Indianmeal moth (IMM). Since it is biologically controlled by B. hebetor venom, therefore in our study, herein for the first time, we report the anti-inflammatory activities of the venom from B. hebetor (BHV). We developed a septic shock mice model for in vivo anti-inflammatory studies and RAW 264.7 cells for in vitro studies. Our results clearly demonstrate that BHV can dose dependently abrogate the nitric oxide (NO) production and suppress the levels of proinflammatory mediators and cytokines without posing any cytotoxicity via the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways.
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spelling pubmed-56820832017-12-06 Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say Saba, Evelyn Shafeeq, Tahir Irfan, Muhammad Lee, Yuan Yee Kwon, Hyuk-Woo Seo, Myung Gi Park, Sang-Joon Lee, Kyeong-Yeoll Rhee, Man Hee Mediators Inflamm Research Article Pest control in the agricultural fields, a major concern globally, is currently achieved through chemical or biological methods. Chemical methods, which leave toxic residue in the produce, are less preferred than biological methods. Venoms injected by stings of various wasps that kill the pest is considered as the examples of the biological method. Although several studies have investigated the biological control of pests through these venoms, very few studies have reported the effects of these venoms on mammalian cells. Bracon hebetor, an ectoparasitoid of the order Hymenoptera, is having a paramount importance in parasitizing various lepidopterous larvae including Plodia interpunctella also called as Indianmeal moth (IMM). Since it is biologically controlled by B. hebetor venom, therefore in our study, herein for the first time, we report the anti-inflammatory activities of the venom from B. hebetor (BHV). We developed a septic shock mice model for in vivo anti-inflammatory studies and RAW 264.7 cells for in vitro studies. Our results clearly demonstrate that BHV can dose dependently abrogate the nitric oxide (NO) production and suppress the levels of proinflammatory mediators and cytokines without posing any cytotoxicity via the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. Hindawi 2017 2017-10-29 /pmc/articles/PMC5682083/ /pubmed/29213193 http://dx.doi.org/10.1155/2017/6978194 Text en Copyright © 2017 Evelyn Saba et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Saba, Evelyn
Shafeeq, Tahir
Irfan, Muhammad
Lee, Yuan Yee
Kwon, Hyuk-Woo
Seo, Myung Gi
Park, Sang-Joon
Lee, Kyeong-Yeoll
Rhee, Man Hee
Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say
title Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say
title_full Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say
title_fullStr Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say
title_full_unstemmed Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say
title_short Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say
title_sort anti-inflammatory activity of crude venom isolated from parasitoid wasp, bracon hebetor say
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682083/
https://www.ncbi.nlm.nih.gov/pubmed/29213193
http://dx.doi.org/10.1155/2017/6978194
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