Cargando…

Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study

OBJECTIVES: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems. MATERIALS AND METHODS: Sixty mandibular first molar teeth were used to prepare the test appara...

Descripción completa

Detalles Bibliográficos
Autores principales: Dagna, Alberto, El Abed, Rashid, Hussain, Sameeha, Abu-Tahun, Ibrahim H, Visai, Livia, Bertoglio, Federico, Bosco, Floriana, Beltrami, Riccardo, Poggio, Claudio, Kim, Hyeon-Cheol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Conservative Dentistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682148/
https://www.ncbi.nlm.nih.gov/pubmed/29142880
http://dx.doi.org/10.5395/rde.2017.42.4.316
_version_ 1783278048179650560
author Dagna, Alberto
El Abed, Rashid
Hussain, Sameeha
Abu-Tahun, Ibrahim H
Visai, Livia
Bertoglio, Federico
Bosco, Floriana
Beltrami, Riccardo
Poggio, Claudio
Kim, Hyeon-Cheol
author_facet Dagna, Alberto
El Abed, Rashid
Hussain, Sameeha
Abu-Tahun, Ibrahim H
Visai, Livia
Bertoglio, Federico
Bosco, Floriana
Beltrami, Riccardo
Poggio, Claudio
Kim, Hyeon-Cheol
author_sort Dagna, Alberto
collection PubMed
description OBJECTIVES: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems. MATERIALS AND METHODS: Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU). RESULTS: The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria. CONCLUSIONS: All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.
format Online
Article
Text
id pubmed-5682148
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The Korean Academy of Conservative Dentistry
record_format MEDLINE/PubMed
spelling pubmed-56821482017-11-15 Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study Dagna, Alberto El Abed, Rashid Hussain, Sameeha Abu-Tahun, Ibrahim H Visai, Livia Bertoglio, Federico Bosco, Floriana Beltrami, Riccardo Poggio, Claudio Kim, Hyeon-Cheol Restor Dent Endod Research Article OBJECTIVES: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems. MATERIALS AND METHODS: Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU). RESULTS: The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria. CONCLUSIONS: All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments. The Korean Academy of Conservative Dentistry 2017-11 2017-10-31 /pmc/articles/PMC5682148/ /pubmed/29142880 http://dx.doi.org/10.5395/rde.2017.42.4.316 Text en Copyright © 2017. The Korean Academy of Conservative Dentistry https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dagna, Alberto
El Abed, Rashid
Hussain, Sameeha
Abu-Tahun, Ibrahim H
Visai, Livia
Bertoglio, Federico
Bosco, Floriana
Beltrami, Riccardo
Poggio, Claudio
Kim, Hyeon-Cheol
Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
title Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
title_full Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
title_fullStr Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
title_full_unstemmed Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
title_short Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
title_sort comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682148/
https://www.ncbi.nlm.nih.gov/pubmed/29142880
http://dx.doi.org/10.5395/rde.2017.42.4.316
work_keys_str_mv AT dagnaalberto comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT elabedrashid comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT hussainsameeha comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT abutahunibrahimh comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT visailivia comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT bertogliofederico comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT boscofloriana comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT beltramiriccardo comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT poggioclaudio comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy
AT kimhyeoncheol comparisonofapicalextrusionofintracanalbacteriabyvariousglidepathestablishingsystemsaninvitrostudy