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Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran

AIM: Shiga toxigenic Escherichia coli (STEC) strains as emerging groups of foodborne pathogens are responsible for most foodborne illnesses. The aim of this study was to determine the antibiotic resistance pattern in STEC isolated from traditional milk products and their molecular characterization....

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Autores principales: Chaleshtori, Farhad Sharafati, Arani, Navid Mazroii, Aghadavod, Esmat, Naseri, Afshin, Chaleshtori, Reza Sharafati
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682273/
https://www.ncbi.nlm.nih.gov/pubmed/29184374
http://dx.doi.org/10.14202/vetworld.2017.1264-1268
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author Chaleshtori, Farhad Sharafati
Arani, Navid Mazroii
Aghadavod, Esmat
Naseri, Afshin
Chaleshtori, Reza Sharafati
author_facet Chaleshtori, Farhad Sharafati
Arani, Navid Mazroii
Aghadavod, Esmat
Naseri, Afshin
Chaleshtori, Reza Sharafati
author_sort Chaleshtori, Farhad Sharafati
collection PubMed
description AIM: Shiga toxigenic Escherichia coli (STEC) strains as emerging groups of foodborne pathogens are responsible for most foodborne illnesses. The aim of this study was to determine the antibiotic resistance pattern in STEC isolated from traditional milk products and their molecular characterization. MATERIALS AND METHODS: A total of 116 samples were randomly purchased from local markets in Kashan, Iran, and evaluated for the occurrence of STEC by culturing and molecular methods. The antibiotic resistance of obtained isolates was determined by Kirby Bauer method. Furthermore, isolates were assayed for the presence of Shiga toxins (stx1 and stx2) and intimin gene (eae). RESULTS: The incidence of E. coli in 60 ice cream, 30 yoghurt, and 26 cheese samples was 8.33%, 10%, and 11.54%, respectively. The findings showed that 11 out of 11 (100%) E. coli had both stx1 and stx2 while eae gene was not found in E. coli isolated of traditional milk products. For E. coli strains carrying stx1 and stx2, highest antibiotic sensitive levels were related to trimethoprim/sulfamethoxazole, norfloxacin, chloramphenicol, and ciprofloxacin, respectively. CONCLUSION: The results showed relationship between the presence of virulence factors and antimicrobial resistance. These results can be used for further studies on STEC as an emerging foodborne pathogen.
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spelling pubmed-56822732017-11-28 Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran Chaleshtori, Farhad Sharafati Arani, Navid Mazroii Aghadavod, Esmat Naseri, Afshin Chaleshtori, Reza Sharafati Vet World Research Article AIM: Shiga toxigenic Escherichia coli (STEC) strains as emerging groups of foodborne pathogens are responsible for most foodborne illnesses. The aim of this study was to determine the antibiotic resistance pattern in STEC isolated from traditional milk products and their molecular characterization. MATERIALS AND METHODS: A total of 116 samples were randomly purchased from local markets in Kashan, Iran, and evaluated for the occurrence of STEC by culturing and molecular methods. The antibiotic resistance of obtained isolates was determined by Kirby Bauer method. Furthermore, isolates were assayed for the presence of Shiga toxins (stx1 and stx2) and intimin gene (eae). RESULTS: The incidence of E. coli in 60 ice cream, 30 yoghurt, and 26 cheese samples was 8.33%, 10%, and 11.54%, respectively. The findings showed that 11 out of 11 (100%) E. coli had both stx1 and stx2 while eae gene was not found in E. coli isolated of traditional milk products. For E. coli strains carrying stx1 and stx2, highest antibiotic sensitive levels were related to trimethoprim/sulfamethoxazole, norfloxacin, chloramphenicol, and ciprofloxacin, respectively. CONCLUSION: The results showed relationship between the presence of virulence factors and antimicrobial resistance. These results can be used for further studies on STEC as an emerging foodborne pathogen. Veterinary World 2017-10 2017-10-24 /pmc/articles/PMC5682273/ /pubmed/29184374 http://dx.doi.org/10.14202/vetworld.2017.1264-1268 Text en Copyright: © Chaleshtori, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Chaleshtori, Farhad Sharafati
Arani, Navid Mazroii
Aghadavod, Esmat
Naseri, Afshin
Chaleshtori, Reza Sharafati
Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran
title Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran
title_full Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran
title_fullStr Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran
title_full_unstemmed Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran
title_short Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran
title_sort molecular characterization of escherichia coli recovered from traditional milk products in kashan, iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682273/
https://www.ncbi.nlm.nih.gov/pubmed/29184374
http://dx.doi.org/10.14202/vetworld.2017.1264-1268
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