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Gene Editing and Crop Improvement Using CRISPR-Cas9 System

Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guideRNA...

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Detalles Bibliográficos
Autores principales: Arora, Leena, Narula, Alka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682324/
https://www.ncbi.nlm.nih.gov/pubmed/29167680
http://dx.doi.org/10.3389/fpls.2017.01932
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author Arora, Leena
Narula, Alka
author_facet Arora, Leena
Narula, Alka
author_sort Arora, Leena
collection PubMed
description Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guideRNA (gRNA) to a specific sequence and the Cas9 endonuclease activity. It has broadened the agricultural research area, bringing in new opportunities to develop novel plant varieties with deletion of detrimental traits or addition of significant characters. This RNA guided genome editing technology is turning out to be a groundbreaking innovation in distinct branches of plant biology. CRISPR technology is constantly advancing including options for various genetic manipulations like generating knockouts; making precise modifications, multiplex genome engineering, and activation and repression of target genes. The review highlights the progression throughout the CRISPR legacy. We have studied the rapid evolution of CRISPR/Cas9 tools with myriad functionalities, capabilities, and specialized applications. Among varied diligences, plant nutritional improvement, enhancement of plant disease resistance and production of drought tolerant plants are reviewed. The review also includes some information on traditional delivery methods of Cas9-gRNA complexes into plant cells and incorporates the advent of CRISPR ribonucleoproteins (RNPs) that came up as a solution to various limitations that prevailed with plasmid-based CRISPR system.
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spelling pubmed-56823242017-11-22 Gene Editing and Crop Improvement Using CRISPR-Cas9 System Arora, Leena Narula, Alka Front Plant Sci Plant Science Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guideRNA (gRNA) to a specific sequence and the Cas9 endonuclease activity. It has broadened the agricultural research area, bringing in new opportunities to develop novel plant varieties with deletion of detrimental traits or addition of significant characters. This RNA guided genome editing technology is turning out to be a groundbreaking innovation in distinct branches of plant biology. CRISPR technology is constantly advancing including options for various genetic manipulations like generating knockouts; making precise modifications, multiplex genome engineering, and activation and repression of target genes. The review highlights the progression throughout the CRISPR legacy. We have studied the rapid evolution of CRISPR/Cas9 tools with myriad functionalities, capabilities, and specialized applications. Among varied diligences, plant nutritional improvement, enhancement of plant disease resistance and production of drought tolerant plants are reviewed. The review also includes some information on traditional delivery methods of Cas9-gRNA complexes into plant cells and incorporates the advent of CRISPR ribonucleoproteins (RNPs) that came up as a solution to various limitations that prevailed with plasmid-based CRISPR system. Frontiers Media S.A. 2017-11-08 /pmc/articles/PMC5682324/ /pubmed/29167680 http://dx.doi.org/10.3389/fpls.2017.01932 Text en Copyright © 2017 Arora and Narula. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Arora, Leena
Narula, Alka
Gene Editing and Crop Improvement Using CRISPR-Cas9 System
title Gene Editing and Crop Improvement Using CRISPR-Cas9 System
title_full Gene Editing and Crop Improvement Using CRISPR-Cas9 System
title_fullStr Gene Editing and Crop Improvement Using CRISPR-Cas9 System
title_full_unstemmed Gene Editing and Crop Improvement Using CRISPR-Cas9 System
title_short Gene Editing and Crop Improvement Using CRISPR-Cas9 System
title_sort gene editing and crop improvement using crispr-cas9 system
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682324/
https://www.ncbi.nlm.nih.gov/pubmed/29167680
http://dx.doi.org/10.3389/fpls.2017.01932
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