Cargando…

A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis

Emerging evidence suggests that microRNA (miRNA) and long noncoding RNA (lncRNA) play important roles in disease development. However, the mechanism underlying mRNA interaction with miRNA and lncRNA in idiopathic pulmonary fibrosis (IPF) remains unknown. This study presents a novel lnc-PCF that prom...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Huizhu, Wang, Bingsi, Zhang, Jinjin, Zhang, Songzi, Wang, Youlei, Zhang, Jie, Lv, Changjun, Song, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682666/
https://www.ncbi.nlm.nih.gov/pubmed/29072702
http://dx.doi.org/10.1038/cddis.2017.500
_version_ 1783278137333776384
author Liu, Huizhu
Wang, Bingsi
Zhang, Jinjin
Zhang, Songzi
Wang, Youlei
Zhang, Jie
Lv, Changjun
Song, Xiaodong
author_facet Liu, Huizhu
Wang, Bingsi
Zhang, Jinjin
Zhang, Songzi
Wang, Youlei
Zhang, Jie
Lv, Changjun
Song, Xiaodong
author_sort Liu, Huizhu
collection PubMed
description Emerging evidence suggests that microRNA (miRNA) and long noncoding RNA (lncRNA) play important roles in disease development. However, the mechanism underlying mRNA interaction with miRNA and lncRNA in idiopathic pulmonary fibrosis (IPF) remains unknown. This study presents a novel lnc-PCF that promotes the proliferation of TGF-β1-activated epithelial cells through the regulation of map3k11 by directly targeting miR-344a-5p during pulmonary fibrogenesis. Bioinformatics and in vitro translation assay were performed to confirm whether or not lnc-PCF is an actual lncRNA. RNA fluorescent in situ hybridization (FISH) and nucleocytoplasmic separation showed that lnc-PCF is mainly expressed in the cytoplasm. Knockdown and knockin of lnc-PCF indicated that lnc-PCF could promote fibrogenesis by regulating the proliferation of epithelial cells activated by TGF-β1 according to the results of xCELLigence real-time cell analysis system, flow cytometry, and western blot analysis. Computational analysis and a dual-luciferase reporter system were used to identify the target gene of miR-344a-5p, whereas RNA pull down, anti-AGO2 RNA immunoprecipitation, and rescue experiments were conducted to confirm the identity of this direct target. Further experiments verified that lnc-PCF promotes the proliferation of activated epithelial cells that were dependent on miR-344a-5p, which exerted its regulatory functions through its target gene map3k11. Finally, adenovirus packaging sh-lnc-PCF was sprayed into rat lung tissues to evaluate the therapeutic effect of lnc-PCF. These findings revealed that lnc-PCF can accelerate pulmonary fibrogenesis by directly targeting miR-344a-5p to regulate map3k11, which may be a potential therapeutic target in IPF.
format Online
Article
Text
id pubmed-5682666
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-56826662017-11-16 A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis Liu, Huizhu Wang, Bingsi Zhang, Jinjin Zhang, Songzi Wang, Youlei Zhang, Jie Lv, Changjun Song, Xiaodong Cell Death Dis Original Article Emerging evidence suggests that microRNA (miRNA) and long noncoding RNA (lncRNA) play important roles in disease development. However, the mechanism underlying mRNA interaction with miRNA and lncRNA in idiopathic pulmonary fibrosis (IPF) remains unknown. This study presents a novel lnc-PCF that promotes the proliferation of TGF-β1-activated epithelial cells through the regulation of map3k11 by directly targeting miR-344a-5p during pulmonary fibrogenesis. Bioinformatics and in vitro translation assay were performed to confirm whether or not lnc-PCF is an actual lncRNA. RNA fluorescent in situ hybridization (FISH) and nucleocytoplasmic separation showed that lnc-PCF is mainly expressed in the cytoplasm. Knockdown and knockin of lnc-PCF indicated that lnc-PCF could promote fibrogenesis by regulating the proliferation of epithelial cells activated by TGF-β1 according to the results of xCELLigence real-time cell analysis system, flow cytometry, and western blot analysis. Computational analysis and a dual-luciferase reporter system were used to identify the target gene of miR-344a-5p, whereas RNA pull down, anti-AGO2 RNA immunoprecipitation, and rescue experiments were conducted to confirm the identity of this direct target. Further experiments verified that lnc-PCF promotes the proliferation of activated epithelial cells that were dependent on miR-344a-5p, which exerted its regulatory functions through its target gene map3k11. Finally, adenovirus packaging sh-lnc-PCF was sprayed into rat lung tissues to evaluate the therapeutic effect of lnc-PCF. These findings revealed that lnc-PCF can accelerate pulmonary fibrogenesis by directly targeting miR-344a-5p to regulate map3k11, which may be a potential therapeutic target in IPF. Nature Publishing Group 2017-10 2017-10-26 /pmc/articles/PMC5682666/ /pubmed/29072702 http://dx.doi.org/10.1038/cddis.2017.500 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Liu, Huizhu
Wang, Bingsi
Zhang, Jinjin
Zhang, Songzi
Wang, Youlei
Zhang, Jie
Lv, Changjun
Song, Xiaodong
A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis
title A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis
title_full A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis
title_fullStr A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis
title_full_unstemmed A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis
title_short A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis
title_sort novel lnc-pcf promotes the proliferation of tgf-β1-activated epithelial cells by targeting mir-344a-5p to regulate map3k11 in pulmonary fibrosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5682666/
https://www.ncbi.nlm.nih.gov/pubmed/29072702
http://dx.doi.org/10.1038/cddis.2017.500
work_keys_str_mv AT liuhuizhu anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT wangbingsi anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT zhangjinjin anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT zhangsongzi anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT wangyoulei anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT zhangjie anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT lvchangjun anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT songxiaodong anovellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT liuhuizhu novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT wangbingsi novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT zhangjinjin novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT zhangsongzi novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT wangyoulei novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT zhangjie novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT lvchangjun novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis
AT songxiaodong novellncpcfpromotestheproliferationoftgfb1activatedepithelialcellsbytargetingmir344a5ptoregulatemap3k11inpulmonaryfibrosis