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Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption

BACKGROUND: The olfactory system enables organisms to detect chemical cues in the environment and can signal the availability of food or the presence of a predator. Appropriate behavioral responses to these chemical cues are therefore important for organismal survival and can influence traits such a...

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Autores principales: Brown, Elizabeth B., Patterson, Cody, Pancoast, Rayanne, Rollmann, Stephanie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683340/
https://www.ncbi.nlm.nih.gov/pubmed/29132294
http://dx.doi.org/10.1186/s12864-017-4233-1
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author Brown, Elizabeth B.
Patterson, Cody
Pancoast, Rayanne
Rollmann, Stephanie M.
author_facet Brown, Elizabeth B.
Patterson, Cody
Pancoast, Rayanne
Rollmann, Stephanie M.
author_sort Brown, Elizabeth B.
collection PubMed
description BACKGROUND: The olfactory system enables organisms to detect chemical cues in the environment and can signal the availability of food or the presence of a predator. Appropriate behavioral responses to these chemical cues are therefore important for organismal survival and can influence traits such as organismal life span and food consumption. However, understanding the genetic mechanisms underlying odor-guided behavior, correlated responses in other traits, and how these constrain or promote their evolution, remain an important challenge. Here, we performed artificial selection for attractive and aversive behavioral responses to four chemical compounds, two aromatics (4-ethylguaiacol and 4-methylphenol) and two esters (methyl hexanoate and ethyl acetate), for thirty generations. RESULTS: Artificial selection for odor-guided behavior revealed symmetrical responses to selection for each of the four chemical compounds. We then investigated whether selection for odor-guided behavior resulted in correlated responses in life history traits and/or food consumption. We found changes in food consumption upon selection for behavioral responses to aromatics. In many cases, lines selected for increased attraction to aromatics showed an increase in food consumption. We then performed RNA sequencing of lines selected for responses to 4-ethylguaiacol to identify candidate genes associated with odor-guided behavior and its impact on food consumption. We identified 91 genes that were differentially expressed among lines, many of which were associated with metabolic processes. RNAi-mediated knockdown of select candidate genes further supports their role in odor-guided behavior and/or food consumption. CONCLUSIONS: This study identifies novel genes underlying variation in odor-guided behavior and further elucidates the genetic mechanisms underlying the interrelationship between olfaction and feeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4233-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-56833402017-11-20 Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption Brown, Elizabeth B. Patterson, Cody Pancoast, Rayanne Rollmann, Stephanie M. BMC Genomics Research Article BACKGROUND: The olfactory system enables organisms to detect chemical cues in the environment and can signal the availability of food or the presence of a predator. Appropriate behavioral responses to these chemical cues are therefore important for organismal survival and can influence traits such as organismal life span and food consumption. However, understanding the genetic mechanisms underlying odor-guided behavior, correlated responses in other traits, and how these constrain or promote their evolution, remain an important challenge. Here, we performed artificial selection for attractive and aversive behavioral responses to four chemical compounds, two aromatics (4-ethylguaiacol and 4-methylphenol) and two esters (methyl hexanoate and ethyl acetate), for thirty generations. RESULTS: Artificial selection for odor-guided behavior revealed symmetrical responses to selection for each of the four chemical compounds. We then investigated whether selection for odor-guided behavior resulted in correlated responses in life history traits and/or food consumption. We found changes in food consumption upon selection for behavioral responses to aromatics. In many cases, lines selected for increased attraction to aromatics showed an increase in food consumption. We then performed RNA sequencing of lines selected for responses to 4-ethylguaiacol to identify candidate genes associated with odor-guided behavior and its impact on food consumption. We identified 91 genes that were differentially expressed among lines, many of which were associated with metabolic processes. RNAi-mediated knockdown of select candidate genes further supports their role in odor-guided behavior and/or food consumption. CONCLUSIONS: This study identifies novel genes underlying variation in odor-guided behavior and further elucidates the genetic mechanisms underlying the interrelationship between olfaction and feeding. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-017-4233-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-11-13 /pmc/articles/PMC5683340/ /pubmed/29132294 http://dx.doi.org/10.1186/s12864-017-4233-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Brown, Elizabeth B.
Patterson, Cody
Pancoast, Rayanne
Rollmann, Stephanie M.
Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption
title Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption
title_full Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption
title_fullStr Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption
title_full_unstemmed Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption
title_short Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption
title_sort artificial selection for odor-guided behavior in drosophila reveals changes in food consumption
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683340/
https://www.ncbi.nlm.nih.gov/pubmed/29132294
http://dx.doi.org/10.1186/s12864-017-4233-1
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