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Quantifying mitochondrial DNA copy number using robust regression to interpret real time PCR results

BACKGROUND: Real time PCR (rtPCR) is a quantitative assay to determine the relative DNA copy number in a sample versus a reference. The [Formula: see text] method is the standard for the analysis of the output data generated by an rtPCR experiment. We developed an alternative based on fitting a robu...

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Detalles Bibliográficos
Autores principales:	Refinetti, Paulo, Warren, David, Morgenthaler, Stephan, Ekstrøm, Per O.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683470/ https://www.ncbi.nlm.nih.gov/pubmed/29132417 http://dx.doi.org/10.1186/s13104-017-2913-1

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