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Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma

BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is a worldwide concern. This study looked at the relationship between the expression of differential proteins and the clinicopathological data and survival rate of ESCC patients to identify potential tumor markers for the growth and metastasis of...

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Autores principales: Zhang, Yan, Feng, Zhiyin, Wang, Weina, Dong, Juanjuan, Gong, Xiaojin, Pu, Hongwei, Chen, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683682/
https://www.ncbi.nlm.nih.gov/pubmed/29099815
http://dx.doi.org/10.12659/MSM.904912
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author Zhang, Yan
Feng, Zhiyin
Wang, Weina
Dong, Juanjuan
Gong, Xiaojin
Pu, Hongwei
Chen, Xiao
author_facet Zhang, Yan
Feng, Zhiyin
Wang, Weina
Dong, Juanjuan
Gong, Xiaojin
Pu, Hongwei
Chen, Xiao
author_sort Zhang, Yan
collection PubMed
description BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is a worldwide concern. This study looked at the relationship between the expression of differential proteins and the clinicopathological data and survival rate of ESCC patients to identify potential tumor markers for the growth and metastasis of ESCC. MATERIAL/METHODS: This study included 162 patients who underwent surgical excision for management of ESCC. Fresh ESCC tissue and adjacent normal tissue specimens were collected. Protein expressions were detected by western blotting. The expression of Hsp27 and P38MAPK were detected by immunohistochemistry in formalin-fixed paraffin embedded primary tissue specimens. RESULTS: The rate of positive Hsp27 and P38MAPK expression in ESCC tissue were higher than in normal esophageal tissue (p<0.05). The expression of P38MAPK was related to the depth of infiltration (p<0.05). The expression of Hsp27 was correlated with lymph node metastasis (p<0.05), but not with age, depth of infiltration, or tumor size. ROC were plotted to estimate the significance of the diagnosis: for Hsp27, AUC=0.735 (p<0.05), for P38MAPK, AUC=0.882 (p<0.05). CONCLUSIONS: The expression of Hsp27 and P38MAPK plays a role in ESCC development. Hsp27 and P38MAPK could be used as prognostic factors in ESCC.
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spelling pubmed-56836822017-11-16 Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma Zhang, Yan Feng, Zhiyin Wang, Weina Dong, Juanjuan Gong, Xiaojin Pu, Hongwei Chen, Xiao Med Sci Monit Clinical Research BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is a worldwide concern. This study looked at the relationship between the expression of differential proteins and the clinicopathological data and survival rate of ESCC patients to identify potential tumor markers for the growth and metastasis of ESCC. MATERIAL/METHODS: This study included 162 patients who underwent surgical excision for management of ESCC. Fresh ESCC tissue and adjacent normal tissue specimens were collected. Protein expressions were detected by western blotting. The expression of Hsp27 and P38MAPK were detected by immunohistochemistry in formalin-fixed paraffin embedded primary tissue specimens. RESULTS: The rate of positive Hsp27 and P38MAPK expression in ESCC tissue were higher than in normal esophageal tissue (p<0.05). The expression of P38MAPK was related to the depth of infiltration (p<0.05). The expression of Hsp27 was correlated with lymph node metastasis (p<0.05), but not with age, depth of infiltration, or tumor size. ROC were plotted to estimate the significance of the diagnosis: for Hsp27, AUC=0.735 (p<0.05), for P38MAPK, AUC=0.882 (p<0.05). CONCLUSIONS: The expression of Hsp27 and P38MAPK plays a role in ESCC development. Hsp27 and P38MAPK could be used as prognostic factors in ESCC. International Scientific Literature, Inc. 2017-11-03 /pmc/articles/PMC5683682/ /pubmed/29099815 http://dx.doi.org/10.12659/MSM.904912 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Clinical Research
Zhang, Yan
Feng, Zhiyin
Wang, Weina
Dong, Juanjuan
Gong, Xiaojin
Pu, Hongwei
Chen, Xiao
Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma
title Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma
title_full Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma
title_fullStr Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma
title_full_unstemmed Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma
title_short Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma
title_sort expression of heat shock protein-27 (hsp27) and p38mapk in esophageal squamous cell carcinoma
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683682/
https://www.ncbi.nlm.nih.gov/pubmed/29099815
http://dx.doi.org/10.12659/MSM.904912
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