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Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure

MIC60/mitofilin constitutes a hetero-oligomeric complex on the inner mitochondrial membranes to maintain crista structure. However, little is known about its physiological functions. Here, by characterizing Drosophila MIC60 mutants, we define its roles in vivo. We discover that MIC60 performs dual f...

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Autores principales: Tsai, Pei-I, Papakyrikos, Amanda M., Hsieh, Chung-Han, Wang, Xinnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683758/
https://www.ncbi.nlm.nih.gov/pubmed/28904209
http://dx.doi.org/10.1091/mbc.E17-03-0177
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author Tsai, Pei-I
Papakyrikos, Amanda M.
Hsieh, Chung-Han
Wang, Xinnan
author_facet Tsai, Pei-I
Papakyrikos, Amanda M.
Hsieh, Chung-Han
Wang, Xinnan
author_sort Tsai, Pei-I
collection PubMed
description MIC60/mitofilin constitutes a hetero-oligomeric complex on the inner mitochondrial membranes to maintain crista structure. However, little is known about its physiological functions. Here, by characterizing Drosophila MIC60 mutants, we define its roles in vivo. We discover that MIC60 performs dual functions to maintain mitochondrial homeostasis. In addition to its canonical role in crista membrane structure, MIC60 regulates mitochondrial motility, likely by influencing protein levels of the outer mitochondrial membrane protein Miro that anchors mitochondria to the microtubule motors. Loss of MIC60 causes loss of Miro and mitochondrial arrest. At a cellular level, loss of MIC60 disrupts synaptic structure and function at the neuromuscular junctions. The dual roles of MIC60 in both mitochondrial crista structure and motility position it as a crucial player for cellular integrity and survival.
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spelling pubmed-56837582018-01-30 Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure Tsai, Pei-I Papakyrikos, Amanda M. Hsieh, Chung-Han Wang, Xinnan Mol Biol Cell Brief Reports MIC60/mitofilin constitutes a hetero-oligomeric complex on the inner mitochondrial membranes to maintain crista structure. However, little is known about its physiological functions. Here, by characterizing Drosophila MIC60 mutants, we define its roles in vivo. We discover that MIC60 performs dual functions to maintain mitochondrial homeostasis. In addition to its canonical role in crista membrane structure, MIC60 regulates mitochondrial motility, likely by influencing protein levels of the outer mitochondrial membrane protein Miro that anchors mitochondria to the microtubule motors. Loss of MIC60 causes loss of Miro and mitochondrial arrest. At a cellular level, loss of MIC60 disrupts synaptic structure and function at the neuromuscular junctions. The dual roles of MIC60 in both mitochondrial crista structure and motility position it as a crucial player for cellular integrity and survival. The American Society for Cell Biology 2017-11-15 /pmc/articles/PMC5683758/ /pubmed/28904209 http://dx.doi.org/10.1091/mbc.E17-03-0177 Text en © 2017 Tsai, Papakyrikos, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Brief Reports
Tsai, Pei-I
Papakyrikos, Amanda M.
Hsieh, Chung-Han
Wang, Xinnan
Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure
title Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure
title_full Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure
title_fullStr Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure
title_full_unstemmed Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure
title_short Drosophila MIC60/mitofilin conducts dual roles in mitochondrial motility and crista structure
title_sort drosophila mic60/mitofilin conducts dual roles in mitochondrial motility and crista structure
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5683758/
https://www.ncbi.nlm.nih.gov/pubmed/28904209
http://dx.doi.org/10.1091/mbc.E17-03-0177
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