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Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro
Three-dimensional aggregation and organ culture methods are critical for recreating in vivo cellular phenomena outside the body. Previously, we used the conventional gas liquid interphase organ culture method to induce complete mouse spermatogenesis. After incorporating microfluidic systems, we achi...
Autores principales: | Komeya, Mitsuru, Hayashi, Kazuaki, Nakamura, Hiroko, Yamanaka, Hiroyuki, Sanjo, Hiroyuki, Kojima, Kazuaki, Sato, Takuya, Yao, Masahiro, Kimura, Hiroshi, Fujii, Teruo, Ogawa, Takehiko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684205/ https://www.ncbi.nlm.nih.gov/pubmed/29133858 http://dx.doi.org/10.1038/s41598-017-15799-3 |
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