Development of the Multiple Gene Knockout System with One-Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps. Alternatively, PCR tailing for high-throughput gene disruption was also developed in S. acidocaldarius,...

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Detalles Bibliográficos
Autores principales: Suzuki, Shoji, Kurosawa, Norio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684531/
https://www.ncbi.nlm.nih.gov/pubmed/29225512
http://dx.doi.org/10.1155/2017/7459310

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684531/
https://www.ncbi.nlm.nih.gov/pubmed/29225512
http://dx.doi.org/10.1155/2017/7459310

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