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Metabolic Differences between Dogs of Different Body Sizes
INTRODUCTION: The domesticated dog, Canis lupus familiaris, has been selectively bred to produce extreme diversity in phenotype and genotype. Dogs have an immense diversity in weight and height. Specific differences in metabolism have not been characterized in small dogs as compared to larger dogs....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684564/ https://www.ncbi.nlm.nih.gov/pubmed/29225968 http://dx.doi.org/10.1155/2017/4535710 |
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author | Middleton, Rondo P. Lacroix, Sebastien Scott-Boyer, Marie-Pier Dordevic, Nikola Kennedy, Adam D. Slusky, Amanda R. Carayol, Jerome Petzinger-Germain, Christina Beloshapka, Alison Kaput, Jim |
author_facet | Middleton, Rondo P. Lacroix, Sebastien Scott-Boyer, Marie-Pier Dordevic, Nikola Kennedy, Adam D. Slusky, Amanda R. Carayol, Jerome Petzinger-Germain, Christina Beloshapka, Alison Kaput, Jim |
author_sort | Middleton, Rondo P. |
collection | PubMed |
description | INTRODUCTION: The domesticated dog, Canis lupus familiaris, has been selectively bred to produce extreme diversity in phenotype and genotype. Dogs have an immense diversity in weight and height. Specific differences in metabolism have not been characterized in small dogs as compared to larger dogs. OBJECTIVES: This study aims to identify metabolic, clinical, and microbiota differences between small and larger dogs. METHODS: Gas chromatography/mass spectrometry, liquid chromatography/tandem mass spectrometry, clinical chemistry analysis, dual-energy X-ray absorptiometry, and 16S pyrosequencing were used to characterize blood metabolic, clinical, and fecal microbiome systems, respectively. Eighty-three canines from seven different breeds, fed the same kibble diet for 5 weeks, were used in the study. RESULTS: 449 metabolites, 16 clinical parameters, and 6 bacteria (at the genus level) were significantly different between small and larger dogs. Hierarchical clustering of the metabolites yielded 8 modules associated with small dog size. CONCLUSION: Small dogs had a lower antioxidant status and differences in circulating amino acids. Some of the amino acid differences could be attributed to differences in microflora. Additionally, analysis of small dog metabolites and clinical parameters reflected a network which strongly associates with kidney function. |
format | Online Article Text |
id | pubmed-5684564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-56845642017-12-10 Metabolic Differences between Dogs of Different Body Sizes Middleton, Rondo P. Lacroix, Sebastien Scott-Boyer, Marie-Pier Dordevic, Nikola Kennedy, Adam D. Slusky, Amanda R. Carayol, Jerome Petzinger-Germain, Christina Beloshapka, Alison Kaput, Jim J Nutr Metab Research Article INTRODUCTION: The domesticated dog, Canis lupus familiaris, has been selectively bred to produce extreme diversity in phenotype and genotype. Dogs have an immense diversity in weight and height. Specific differences in metabolism have not been characterized in small dogs as compared to larger dogs. OBJECTIVES: This study aims to identify metabolic, clinical, and microbiota differences between small and larger dogs. METHODS: Gas chromatography/mass spectrometry, liquid chromatography/tandem mass spectrometry, clinical chemistry analysis, dual-energy X-ray absorptiometry, and 16S pyrosequencing were used to characterize blood metabolic, clinical, and fecal microbiome systems, respectively. Eighty-three canines from seven different breeds, fed the same kibble diet for 5 weeks, were used in the study. RESULTS: 449 metabolites, 16 clinical parameters, and 6 bacteria (at the genus level) were significantly different between small and larger dogs. Hierarchical clustering of the metabolites yielded 8 modules associated with small dog size. CONCLUSION: Small dogs had a lower antioxidant status and differences in circulating amino acids. Some of the amino acid differences could be attributed to differences in microflora. Additionally, analysis of small dog metabolites and clinical parameters reflected a network which strongly associates with kidney function. Hindawi 2017 2017-10-26 /pmc/articles/PMC5684564/ /pubmed/29225968 http://dx.doi.org/10.1155/2017/4535710 Text en Copyright © 2017 Rondo P. Middleton et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Middleton, Rondo P. Lacroix, Sebastien Scott-Boyer, Marie-Pier Dordevic, Nikola Kennedy, Adam D. Slusky, Amanda R. Carayol, Jerome Petzinger-Germain, Christina Beloshapka, Alison Kaput, Jim Metabolic Differences between Dogs of Different Body Sizes |
title | Metabolic Differences between Dogs of Different Body Sizes |
title_full | Metabolic Differences between Dogs of Different Body Sizes |
title_fullStr | Metabolic Differences between Dogs of Different Body Sizes |
title_full_unstemmed | Metabolic Differences between Dogs of Different Body Sizes |
title_short | Metabolic Differences between Dogs of Different Body Sizes |
title_sort | metabolic differences between dogs of different body sizes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684564/ https://www.ncbi.nlm.nih.gov/pubmed/29225968 http://dx.doi.org/10.1155/2017/4535710 |
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