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Metabolic Differences between Dogs of Different Body Sizes

INTRODUCTION: The domesticated dog, Canis lupus familiaris, has been selectively bred to produce extreme diversity in phenotype and genotype. Dogs have an immense diversity in weight and height. Specific differences in metabolism have not been characterized in small dogs as compared to larger dogs....

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Autores principales: Middleton, Rondo P., Lacroix, Sebastien, Scott-Boyer, Marie-Pier, Dordevic, Nikola, Kennedy, Adam D., Slusky, Amanda R., Carayol, Jerome, Petzinger-Germain, Christina, Beloshapka, Alison, Kaput, Jim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684564/
https://www.ncbi.nlm.nih.gov/pubmed/29225968
http://dx.doi.org/10.1155/2017/4535710
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author Middleton, Rondo P.
Lacroix, Sebastien
Scott-Boyer, Marie-Pier
Dordevic, Nikola
Kennedy, Adam D.
Slusky, Amanda R.
Carayol, Jerome
Petzinger-Germain, Christina
Beloshapka, Alison
Kaput, Jim
author_facet Middleton, Rondo P.
Lacroix, Sebastien
Scott-Boyer, Marie-Pier
Dordevic, Nikola
Kennedy, Adam D.
Slusky, Amanda R.
Carayol, Jerome
Petzinger-Germain, Christina
Beloshapka, Alison
Kaput, Jim
author_sort Middleton, Rondo P.
collection PubMed
description INTRODUCTION: The domesticated dog, Canis lupus familiaris, has been selectively bred to produce extreme diversity in phenotype and genotype. Dogs have an immense diversity in weight and height. Specific differences in metabolism have not been characterized in small dogs as compared to larger dogs. OBJECTIVES: This study aims to identify metabolic, clinical, and microbiota differences between small and larger dogs. METHODS: Gas chromatography/mass spectrometry, liquid chromatography/tandem mass spectrometry, clinical chemistry analysis, dual-energy X-ray absorptiometry, and 16S pyrosequencing were used to characterize blood metabolic, clinical, and fecal microbiome systems, respectively. Eighty-three canines from seven different breeds, fed the same kibble diet for 5 weeks, were used in the study. RESULTS: 449 metabolites, 16 clinical parameters, and 6 bacteria (at the genus level) were significantly different between small and larger dogs. Hierarchical clustering of the metabolites yielded 8 modules associated with small dog size. CONCLUSION: Small dogs had a lower antioxidant status and differences in circulating amino acids. Some of the amino acid differences could be attributed to differences in microflora. Additionally, analysis of small dog metabolites and clinical parameters reflected a network which strongly associates with kidney function.
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spelling pubmed-56845642017-12-10 Metabolic Differences between Dogs of Different Body Sizes Middleton, Rondo P. Lacroix, Sebastien Scott-Boyer, Marie-Pier Dordevic, Nikola Kennedy, Adam D. Slusky, Amanda R. Carayol, Jerome Petzinger-Germain, Christina Beloshapka, Alison Kaput, Jim J Nutr Metab Research Article INTRODUCTION: The domesticated dog, Canis lupus familiaris, has been selectively bred to produce extreme diversity in phenotype and genotype. Dogs have an immense diversity in weight and height. Specific differences in metabolism have not been characterized in small dogs as compared to larger dogs. OBJECTIVES: This study aims to identify metabolic, clinical, and microbiota differences between small and larger dogs. METHODS: Gas chromatography/mass spectrometry, liquid chromatography/tandem mass spectrometry, clinical chemistry analysis, dual-energy X-ray absorptiometry, and 16S pyrosequencing were used to characterize blood metabolic, clinical, and fecal microbiome systems, respectively. Eighty-three canines from seven different breeds, fed the same kibble diet for 5 weeks, were used in the study. RESULTS: 449 metabolites, 16 clinical parameters, and 6 bacteria (at the genus level) were significantly different between small and larger dogs. Hierarchical clustering of the metabolites yielded 8 modules associated with small dog size. CONCLUSION: Small dogs had a lower antioxidant status and differences in circulating amino acids. Some of the amino acid differences could be attributed to differences in microflora. Additionally, analysis of small dog metabolites and clinical parameters reflected a network which strongly associates with kidney function. Hindawi 2017 2017-10-26 /pmc/articles/PMC5684564/ /pubmed/29225968 http://dx.doi.org/10.1155/2017/4535710 Text en Copyright © 2017 Rondo P. Middleton et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Middleton, Rondo P.
Lacroix, Sebastien
Scott-Boyer, Marie-Pier
Dordevic, Nikola
Kennedy, Adam D.
Slusky, Amanda R.
Carayol, Jerome
Petzinger-Germain, Christina
Beloshapka, Alison
Kaput, Jim
Metabolic Differences between Dogs of Different Body Sizes
title Metabolic Differences between Dogs of Different Body Sizes
title_full Metabolic Differences between Dogs of Different Body Sizes
title_fullStr Metabolic Differences between Dogs of Different Body Sizes
title_full_unstemmed Metabolic Differences between Dogs of Different Body Sizes
title_short Metabolic Differences between Dogs of Different Body Sizes
title_sort metabolic differences between dogs of different body sizes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684564/
https://www.ncbi.nlm.nih.gov/pubmed/29225968
http://dx.doi.org/10.1155/2017/4535710
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