New Transcriptional Reporters to Quantify and Monitor PPARγ Activity

The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter ge...

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Detalles Bibliográficos
Autores principales: Degrelle, Séverine A., Shoaito, Hussein, Fournier, Thierry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684601/
https://www.ncbi.nlm.nih.gov/pubmed/29225614
http://dx.doi.org/10.1155/2017/6139107
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author Degrelle, Séverine A.
Shoaito, Hussein
Fournier, Thierry
author_facet Degrelle, Séverine A.
Shoaito, Hussein
Fournier, Thierry
author_sort Degrelle, Séverine A.
collection PubMed
description The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ's potential ligands.
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spelling pubmed-56846012017-12-10 New Transcriptional Reporters to Quantify and Monitor PPARγ Activity Degrelle, Séverine A. Shoaito, Hussein Fournier, Thierry PPAR Res Research Article The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ's potential ligands. Hindawi 2017 2017-11-01 /pmc/articles/PMC5684601/ /pubmed/29225614 http://dx.doi.org/10.1155/2017/6139107 Text en Copyright © 2017 Séverine A. Degrelle et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Degrelle, Séverine A.
Shoaito, Hussein
Fournier, Thierry
New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
title New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
title_full New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
title_fullStr New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
title_full_unstemmed New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
title_short New Transcriptional Reporters to Quantify and Monitor PPARγ Activity
title_sort new transcriptional reporters to quantify and monitor pparγ activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5684601/
https://www.ncbi.nlm.nih.gov/pubmed/29225614
http://dx.doi.org/10.1155/2017/6139107
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