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Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring

An increase in imprinting disorders in children conceived though assisted reproductive technologies (ARTs) has been the subject of several reports. The transmission of imprinting errors from the sperm of infertile fathers is believed to be a possible reason for the increased occurrence of these diso...

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Autores principales: Tang, Li, Liu, Zichao, Zhang, Ruopeng, Su, Cunmei, Yang, Wenjuan, Yao, Youlin, Zhao, Shuhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685618/
https://www.ncbi.nlm.nih.gov/pubmed/29136648
http://dx.doi.org/10.1371/journal.pone.0187869
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author Tang, Li
Liu, Zichao
Zhang, Ruopeng
Su, Cunmei
Yang, Wenjuan
Yao, Youlin
Zhao, Shuhua
author_facet Tang, Li
Liu, Zichao
Zhang, Ruopeng
Su, Cunmei
Yang, Wenjuan
Yao, Youlin
Zhao, Shuhua
author_sort Tang, Li
collection PubMed
description An increase in imprinting disorders in children conceived though assisted reproductive technologies (ARTs) has been the subject of several reports. The transmission of imprinting errors from the sperm of infertile fathers is believed to be a possible reason for the increased occurrence of these disorders. However, whether the imprinting alterations in sperm affect ART outcomes and the imprinting of offspring is unclear. In the current study, we analyzed the methylation of H19, SNRPN and KCNQ1OT1 by pyrosequencing sperm samples from 97 infertile patients and 31 proven fertile males as well as cord blood samples from 13 infantswho were conceived by infertile parents through intracytoplasmic sperm injection (ICSI) and 30 healthy newborns who were conceived naturally. After four cases were excluded owing to the lack of a sequencing signal, the infertile patients were subgrouped into normal (69 cases) and abnormal (24 cases) imprinting groups according to the reference range set by the control group. Between the groups, there were no significant differences in ART outcomes. Significantly different levels of methylation were detected in H19, but none of the imprinted genes were determined to be outside of the methylation reference range set by the values derived from the naturally conceived controls. Three CpG loci were found to be significantly hypomethylated in the maternally imprinted gene KCNQ1OT1 in two patients from the abnormal imprinting group, none of which were caused by sperm imprinting errors. In addition, the paternal H19 gene exhibited discrepant methylation patterns between the sperm controls and the cord blood controls. Our data suggest that increased imprinting errors in the sperm of infertile patients do not have an obvious influence on ART outcomes or the imprinting of offspring.
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spelling pubmed-56856182017-11-30 Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring Tang, Li Liu, Zichao Zhang, Ruopeng Su, Cunmei Yang, Wenjuan Yao, Youlin Zhao, Shuhua PLoS One Research Article An increase in imprinting disorders in children conceived though assisted reproductive technologies (ARTs) has been the subject of several reports. The transmission of imprinting errors from the sperm of infertile fathers is believed to be a possible reason for the increased occurrence of these disorders. However, whether the imprinting alterations in sperm affect ART outcomes and the imprinting of offspring is unclear. In the current study, we analyzed the methylation of H19, SNRPN and KCNQ1OT1 by pyrosequencing sperm samples from 97 infertile patients and 31 proven fertile males as well as cord blood samples from 13 infantswho were conceived by infertile parents through intracytoplasmic sperm injection (ICSI) and 30 healthy newborns who were conceived naturally. After four cases were excluded owing to the lack of a sequencing signal, the infertile patients were subgrouped into normal (69 cases) and abnormal (24 cases) imprinting groups according to the reference range set by the control group. Between the groups, there were no significant differences in ART outcomes. Significantly different levels of methylation were detected in H19, but none of the imprinted genes were determined to be outside of the methylation reference range set by the values derived from the naturally conceived controls. Three CpG loci were found to be significantly hypomethylated in the maternally imprinted gene KCNQ1OT1 in two patients from the abnormal imprinting group, none of which were caused by sperm imprinting errors. In addition, the paternal H19 gene exhibited discrepant methylation patterns between the sperm controls and the cord blood controls. Our data suggest that increased imprinting errors in the sperm of infertile patients do not have an obvious influence on ART outcomes or the imprinting of offspring. Public Library of Science 2017-11-14 /pmc/articles/PMC5685618/ /pubmed/29136648 http://dx.doi.org/10.1371/journal.pone.0187869 Text en © 2017 Tang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tang, Li
Liu, Zichao
Zhang, Ruopeng
Su, Cunmei
Yang, Wenjuan
Yao, Youlin
Zhao, Shuhua
Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring
title Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring
title_full Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring
title_fullStr Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring
title_full_unstemmed Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring
title_short Imprinting alterations in sperm may not significantly influence ART outcomes and imprinting patterns in the cord blood of offspring
title_sort imprinting alterations in sperm may not significantly influence art outcomes and imprinting patterns in the cord blood of offspring
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685618/
https://www.ncbi.nlm.nih.gov/pubmed/29136648
http://dx.doi.org/10.1371/journal.pone.0187869
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