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Identification of metastasis-associated microRNAs in serum from rectal cancer patients
MicroRNAs (miRNAs) are promising prognostic and diagnostic biomarkers due to their high stability in blood. Here we investigate the expression of miRNAs and other noncoding (nc) RNAs in serum of rectal cancer patients. Serum from 96 rectal cancer patients was profiled using small RNA sequencing and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685733/ https://www.ncbi.nlm.nih.gov/pubmed/29163812 http://dx.doi.org/10.18632/oncotarget.21412 |
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author | Mjelle, Robin Sellæg, Kjersti Sætrom, Pål Thommesen, Liv Sjursen, Wenche Hofsli, Eva |
author_facet | Mjelle, Robin Sellæg, Kjersti Sætrom, Pål Thommesen, Liv Sjursen, Wenche Hofsli, Eva |
author_sort | Mjelle, Robin |
collection | PubMed |
description | MicroRNAs (miRNAs) are promising prognostic and diagnostic biomarkers due to their high stability in blood. Here we investigate the expression of miRNAs and other noncoding (nc) RNAs in serum of rectal cancer patients. Serum from 96 rectal cancer patients was profiled using small RNA sequencing and expression of small RNAs was correlated with the clinicopathological characteristics of the patients. Multiple classes of RNAs were detected, including miRNAs and fragments of tRNAs, snoRNAs, long ncRNAs, and other classes of RNAs. Several miRNAs, miRNA variants (isomiRs) and other ncRNAs were differentially expressed between Stage IV and Stage I-III rectal cancer patients, including several members of the miR-320 family. Furthermore, we show that high expression of miR-320d as well as one tRNA fragment is associated with poor survival. We also show that several miRNAs and isomiRs are differentially expressed between patients receiving preoperative chemoradiotherapy and patients who did not receive any treatment before serum collection. In summary, our study shows that the expression of miRNAs and other small ncRNAs in serum may be used to predict distant metastasis and survival in rectal cancer. |
format | Online Article Text |
id | pubmed-5685733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56857332017-11-21 Identification of metastasis-associated microRNAs in serum from rectal cancer patients Mjelle, Robin Sellæg, Kjersti Sætrom, Pål Thommesen, Liv Sjursen, Wenche Hofsli, Eva Oncotarget Research Paper MicroRNAs (miRNAs) are promising prognostic and diagnostic biomarkers due to their high stability in blood. Here we investigate the expression of miRNAs and other noncoding (nc) RNAs in serum of rectal cancer patients. Serum from 96 rectal cancer patients was profiled using small RNA sequencing and expression of small RNAs was correlated with the clinicopathological characteristics of the patients. Multiple classes of RNAs were detected, including miRNAs and fragments of tRNAs, snoRNAs, long ncRNAs, and other classes of RNAs. Several miRNAs, miRNA variants (isomiRs) and other ncRNAs were differentially expressed between Stage IV and Stage I-III rectal cancer patients, including several members of the miR-320 family. Furthermore, we show that high expression of miR-320d as well as one tRNA fragment is associated with poor survival. We also show that several miRNAs and isomiRs are differentially expressed between patients receiving preoperative chemoradiotherapy and patients who did not receive any treatment before serum collection. In summary, our study shows that the expression of miRNAs and other small ncRNAs in serum may be used to predict distant metastasis and survival in rectal cancer. Impact Journals LLC 2017-09-30 /pmc/articles/PMC5685733/ /pubmed/29163812 http://dx.doi.org/10.18632/oncotarget.21412 Text en Copyright: © 2017 Mjelle et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Mjelle, Robin Sellæg, Kjersti Sætrom, Pål Thommesen, Liv Sjursen, Wenche Hofsli, Eva Identification of metastasis-associated microRNAs in serum from rectal cancer patients |
title | Identification of metastasis-associated microRNAs in serum from rectal cancer patients |
title_full | Identification of metastasis-associated microRNAs in serum from rectal cancer patients |
title_fullStr | Identification of metastasis-associated microRNAs in serum from rectal cancer patients |
title_full_unstemmed | Identification of metastasis-associated microRNAs in serum from rectal cancer patients |
title_short | Identification of metastasis-associated microRNAs in serum from rectal cancer patients |
title_sort | identification of metastasis-associated micrornas in serum from rectal cancer patients |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685733/ https://www.ncbi.nlm.nih.gov/pubmed/29163812 http://dx.doi.org/10.18632/oncotarget.21412 |
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