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Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development
BACKGROUND: The identification of biomarkers of post-traumatic osteoarthritis (PTOA) progression is of clinical importance. The aims of this study were: (1) to assess the abilities of various soluble proinflammatory mediators in plasma to distinguish patients with knee PTOA from controls; (2) to det...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685991/ https://www.ncbi.nlm.nih.gov/pubmed/29058227 http://dx.doi.org/10.1007/s10195-017-0473-8 |
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author | Panina, Svetlana B. Krolevets, Igor V. Milyutina, Natalia P. Sagakyants, Alexander B. Kornienko, Igor V. Ananyan, Anzhelika A. Zabrodin, Mikhail A. Plotnikov, Andrey A. Vnukov, Valeriy V. |
author_facet | Panina, Svetlana B. Krolevets, Igor V. Milyutina, Natalia P. Sagakyants, Alexander B. Kornienko, Igor V. Ananyan, Anzhelika A. Zabrodin, Mikhail A. Plotnikov, Andrey A. Vnukov, Valeriy V. |
author_sort | Panina, Svetlana B. |
collection | PubMed |
description | BACKGROUND: The identification of biomarkers of post-traumatic osteoarthritis (PTOA) progression is of clinical importance. The aims of this study were: (1) to assess the abilities of various soluble proinflammatory mediators in plasma to distinguish patients with knee PTOA from controls; (2) to determine the correlations between the mediators in plasma and those mediators in synovial fluid (SF); and (3) to explore the associations of the mediators with radiographic PTOA severity. MATERIALS AND METHODS: The concentrations of IL-1β, IL-6, IL-18, TNFα, and leptin were measured using ELISA. Nitric oxide was determined as nitrite/nitrate (NO(x)) using the Griess reaction. RESULTS: We included 171 subjects (134 PTOA patients and 37 controls) and excluded patients with rheumatoid arthritis or gout. The ROC curve of plasma NO(x) had the highest AUC, a specificity of 100%, and a sensitivity of 84.4%. The combination of IL-6 and leptin proved to be the most discriminatory, with an AUC value of 0.933, a specificity of 96.7%, and a sensitivity of 85.7%. The levels of NO(x), IL-6, IL-18, and leptin in plasma were significantly correlated with their levels in SF. Leptin levels in both plasma (p = 0.036) and SF (p = 0.041) and the synovial IL-18 level (p = 0.045) were correlated with the Kellgren–Lawrence (KL) grade. Early-stage PTOA (KL 1–2) was associated with a high concentration of IL-1β in plasma before and after (OR 6.235, 95% CI 1.362 to 28.552, p = 0.018) adjusting for age, gender, and BMI. CONCLUSIONS: Circulating NO(x) level and a combination of IL-6 and leptin permitted the strongest discrimination of patients with PTOA from controls. PTOA severity was correlated with leptin levels in plasma and SF and with the synovial IL-18 level. Early PTOA was associated with the circulating level of IL-1β. LEVEL OF EVIDENCE: III (case–control study). |
format | Online Article Text |
id | pubmed-5685991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-56859912017-11-28 Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development Panina, Svetlana B. Krolevets, Igor V. Milyutina, Natalia P. Sagakyants, Alexander B. Kornienko, Igor V. Ananyan, Anzhelika A. Zabrodin, Mikhail A. Plotnikov, Andrey A. Vnukov, Valeriy V. J Orthop Traumatol Original Article BACKGROUND: The identification of biomarkers of post-traumatic osteoarthritis (PTOA) progression is of clinical importance. The aims of this study were: (1) to assess the abilities of various soluble proinflammatory mediators in plasma to distinguish patients with knee PTOA from controls; (2) to determine the correlations between the mediators in plasma and those mediators in synovial fluid (SF); and (3) to explore the associations of the mediators with radiographic PTOA severity. MATERIALS AND METHODS: The concentrations of IL-1β, IL-6, IL-18, TNFα, and leptin were measured using ELISA. Nitric oxide was determined as nitrite/nitrate (NO(x)) using the Griess reaction. RESULTS: We included 171 subjects (134 PTOA patients and 37 controls) and excluded patients with rheumatoid arthritis or gout. The ROC curve of plasma NO(x) had the highest AUC, a specificity of 100%, and a sensitivity of 84.4%. The combination of IL-6 and leptin proved to be the most discriminatory, with an AUC value of 0.933, a specificity of 96.7%, and a sensitivity of 85.7%. The levels of NO(x), IL-6, IL-18, and leptin in plasma were significantly correlated with their levels in SF. Leptin levels in both plasma (p = 0.036) and SF (p = 0.041) and the synovial IL-18 level (p = 0.045) were correlated with the Kellgren–Lawrence (KL) grade. Early-stage PTOA (KL 1–2) was associated with a high concentration of IL-1β in plasma before and after (OR 6.235, 95% CI 1.362 to 28.552, p = 0.018) adjusting for age, gender, and BMI. CONCLUSIONS: Circulating NO(x) level and a combination of IL-6 and leptin permitted the strongest discrimination of patients with PTOA from controls. PTOA severity was correlated with leptin levels in plasma and SF and with the synovial IL-18 level. Early PTOA was associated with the circulating level of IL-1β. LEVEL OF EVIDENCE: III (case–control study). Springer International Publishing 2017-10-20 2017-12 /pmc/articles/PMC5685991/ /pubmed/29058227 http://dx.doi.org/10.1007/s10195-017-0473-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Panina, Svetlana B. Krolevets, Igor V. Milyutina, Natalia P. Sagakyants, Alexander B. Kornienko, Igor V. Ananyan, Anzhelika A. Zabrodin, Mikhail A. Plotnikov, Andrey A. Vnukov, Valeriy V. Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development |
title | Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development |
title_full | Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development |
title_fullStr | Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development |
title_full_unstemmed | Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development |
title_short | Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development |
title_sort | circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685991/ https://www.ncbi.nlm.nih.gov/pubmed/29058227 http://dx.doi.org/10.1007/s10195-017-0473-8 |
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