Rapid Purification of Human Bispecific Antibodies via Selective Modulation of Protein A Binding

Methods to rapidly generate high quality bispecific antibodies (BsAb) having normal half-lives are critical for therapeutic programs. Here, we identify 3 mutations (T307P, L309Q, and Q311R or “TLQ”) in the Fc region of human IgG1 which disrupt interaction with protein A while enhancing interaction w...

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Detalles Bibliográficos
Autores principales: Zwolak, Adam, Leettola, Catherine N., Tam, Susan H., Goulet, Dennis R., Derebe, Mehabaw G., Pardinas, Jose R., Zheng, Songmao, Decker, Rose, Emmell, Eva, Chiu, Mark L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686111/
https://www.ncbi.nlm.nih.gov/pubmed/29138497
http://dx.doi.org/10.1038/s41598-017-15748-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686111/
https://www.ncbi.nlm.nih.gov/pubmed/29138497
http://dx.doi.org/10.1038/s41598-017-15748-0

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