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Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development
Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here,...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686188/ https://www.ncbi.nlm.nih.gov/pubmed/29138437 http://dx.doi.org/10.1038/s41598-017-15781-z |
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author | Saini, Ekta Zeeshan, Mohammad Brady, Declan Pandey, Rajan Kaiser, Gesine Koreny, Ludek Kumar, Pradeep Thakur, Vandana Tatiya, Shreyansh Katris, Nicholas J. Limenitakis, Rebecca Stanway Kaur, Inderjeet Green, Judith L. Bottrill, Andrew R. Guttery, David S. Waller, Ross F. Heussler, Volker Holder, Anthony A. Mohmmed, Asif Malhotra, Pawan Tewari, Rita |
author_facet | Saini, Ekta Zeeshan, Mohammad Brady, Declan Pandey, Rajan Kaiser, Gesine Koreny, Ludek Kumar, Pradeep Thakur, Vandana Tatiya, Shreyansh Katris, Nicholas J. Limenitakis, Rebecca Stanway Kaur, Inderjeet Green, Judith L. Bottrill, Andrew R. Guttery, David S. Waller, Ross F. Heussler, Volker Holder, Anthony A. Mohmmed, Asif Malhotra, Pawan Tewari, Rita |
author_sort | Saini, Ekta |
collection | PubMed |
description | Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here, we examine the location, protein interactome and function of PhIL1, an IMC-associated protein on the motile and invasive stages of both human and rodent parasites. We show that PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages. Proteins interacting with PhIL1 were identified, showing that PhIL1 was bound to only some proteins present in the glideosome motor complex (GAP50, GAPM1–3) of both P. falciparum and P. berghei. Analysis of PhIL1 function using gene targeting approaches indicated that the protein is required for both asexual and sexual stages of development. In conclusion, we show that PhIL1 is required for development of all zoite stages of Plasmodium and it is part of a novel protein complex with an overall composition overlapping with but different to that of the glideosome. |
format | Online Article Text |
id | pubmed-5686188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56861882017-11-21 Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development Saini, Ekta Zeeshan, Mohammad Brady, Declan Pandey, Rajan Kaiser, Gesine Koreny, Ludek Kumar, Pradeep Thakur, Vandana Tatiya, Shreyansh Katris, Nicholas J. Limenitakis, Rebecca Stanway Kaur, Inderjeet Green, Judith L. Bottrill, Andrew R. Guttery, David S. Waller, Ross F. Heussler, Volker Holder, Anthony A. Mohmmed, Asif Malhotra, Pawan Tewari, Rita Sci Rep Article Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyosin-mediated motility and host cell invasion. Here, we examine the location, protein interactome and function of PhIL1, an IMC-associated protein on the motile and invasive stages of both human and rodent parasites. We show that PhIL1 is located in the IMC in all three invasive (merozoite, ookinete-, and sporozoite) stages of development, as well as in the male gametocyte and locates both at the apical and basal ends of ookinete and sporozoite stages. Proteins interacting with PhIL1 were identified, showing that PhIL1 was bound to only some proteins present in the glideosome motor complex (GAP50, GAPM1–3) of both P. falciparum and P. berghei. Analysis of PhIL1 function using gene targeting approaches indicated that the protein is required for both asexual and sexual stages of development. In conclusion, we show that PhIL1 is required for development of all zoite stages of Plasmodium and it is part of a novel protein complex with an overall composition overlapping with but different to that of the glideosome. Nature Publishing Group UK 2017-11-14 /pmc/articles/PMC5686188/ /pubmed/29138437 http://dx.doi.org/10.1038/s41598-017-15781-z Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Saini, Ekta Zeeshan, Mohammad Brady, Declan Pandey, Rajan Kaiser, Gesine Koreny, Ludek Kumar, Pradeep Thakur, Vandana Tatiya, Shreyansh Katris, Nicholas J. Limenitakis, Rebecca Stanway Kaur, Inderjeet Green, Judith L. Bottrill, Andrew R. Guttery, David S. Waller, Ross F. Heussler, Volker Holder, Anthony A. Mohmmed, Asif Malhotra, Pawan Tewari, Rita Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development |
title | Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development |
title_full | Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development |
title_fullStr | Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development |
title_full_unstemmed | Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development |
title_short | Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development |
title_sort | photosensitized ina-labelled protein 1 (phil1) is novel component of the inner membrane complex and is required for plasmodium parasite development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686188/ https://www.ncbi.nlm.nih.gov/pubmed/29138437 http://dx.doi.org/10.1038/s41598-017-15781-z |
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