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Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma

Despite diagnostic and therapeutic advances, the 5-year survival rate of oral squamous cell carcinoma (OSCC) remains between 70–80% due to recurrences and secondary metastases to cervical lymph nodes. It is difficult to find these recurrences and metastases postoperatively, thus, careful follow-up i...

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Autores principales: Kitamura, Ryoji, Toyoshima, Takeshi, Tanaka, Hideaki, Kawano, Shintaro, Matsubara, Ryota, Goto, Yuichi, Jinno, Teppei, Maruse, Yasuyuki, Oobu, Kazunari, Nakamura, Seiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686526/
https://www.ncbi.nlm.nih.gov/pubmed/29163698
http://dx.doi.org/10.3892/ol.2017.7066
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author Kitamura, Ryoji
Toyoshima, Takeshi
Tanaka, Hideaki
Kawano, Shintaro
Matsubara, Ryota
Goto, Yuichi
Jinno, Teppei
Maruse, Yasuyuki
Oobu, Kazunari
Nakamura, Seiji
author_facet Kitamura, Ryoji
Toyoshima, Takeshi
Tanaka, Hideaki
Kawano, Shintaro
Matsubara, Ryota
Goto, Yuichi
Jinno, Teppei
Maruse, Yasuyuki
Oobu, Kazunari
Nakamura, Seiji
author_sort Kitamura, Ryoji
collection PubMed
description Despite diagnostic and therapeutic advances, the 5-year survival rate of oral squamous cell carcinoma (OSCC) remains between 70–80% due to recurrences and secondary metastases to cervical lymph nodes. It is difficult to find these recurrences and metastases postoperatively, thus, careful follow-up is recommended. Cytokeratins (CKs) are intermediate filaments of the cytoskeleton and candidate prognostic biomarkers for OSCC, as they are overexpressed in OSCC compared with normal mucosa. The aim of the present study was to determine the relative levels of occurrence of 3 CK mRNA (CK17, CK19, CK20) transcripts in peripheral blood mononuclear cells (PBMC) using reverse transcription-quantitative polymerase chain reaction. The study comprised pre- and post-operative PBMC samples from 19 OSCC patients. In the good-prognosis group, 10 of 13 patients demonstrated reduced CK17 mRNA expression post-operatively, compared with pre-operative samples, conversely, only 3 of 6 patients in the poor-prognosis group had reduced post-operative CK17 mRNA expression. This difference was statistically significant (P<0.01). The disease-free survival rate of the group with reduced post-operative CK17 mRNA expression was significantly increased compared with the elevated CK17 mRNA group (P<0.01); however, the overall survival rates of the two groups were not significantly different. Neither CK19 mRNA nor CK20 mRNA were significantly expressed in the PBMC of OSCC patients. Overall, CK17 mRNA expression may be a useful prognostic biomarker for OSCC.
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spelling pubmed-56865262017-11-21 Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma Kitamura, Ryoji Toyoshima, Takeshi Tanaka, Hideaki Kawano, Shintaro Matsubara, Ryota Goto, Yuichi Jinno, Teppei Maruse, Yasuyuki Oobu, Kazunari Nakamura, Seiji Oncol Lett Articles Despite diagnostic and therapeutic advances, the 5-year survival rate of oral squamous cell carcinoma (OSCC) remains between 70–80% due to recurrences and secondary metastases to cervical lymph nodes. It is difficult to find these recurrences and metastases postoperatively, thus, careful follow-up is recommended. Cytokeratins (CKs) are intermediate filaments of the cytoskeleton and candidate prognostic biomarkers for OSCC, as they are overexpressed in OSCC compared with normal mucosa. The aim of the present study was to determine the relative levels of occurrence of 3 CK mRNA (CK17, CK19, CK20) transcripts in peripheral blood mononuclear cells (PBMC) using reverse transcription-quantitative polymerase chain reaction. The study comprised pre- and post-operative PBMC samples from 19 OSCC patients. In the good-prognosis group, 10 of 13 patients demonstrated reduced CK17 mRNA expression post-operatively, compared with pre-operative samples, conversely, only 3 of 6 patients in the poor-prognosis group had reduced post-operative CK17 mRNA expression. This difference was statistically significant (P<0.01). The disease-free survival rate of the group with reduced post-operative CK17 mRNA expression was significantly increased compared with the elevated CK17 mRNA group (P<0.01); however, the overall survival rates of the two groups were not significantly different. Neither CK19 mRNA nor CK20 mRNA were significantly expressed in the PBMC of OSCC patients. Overall, CK17 mRNA expression may be a useful prognostic biomarker for OSCC. D.A. Spandidos 2017-12 2017-09-26 /pmc/articles/PMC5686526/ /pubmed/29163698 http://dx.doi.org/10.3892/ol.2017.7066 Text en Copyright: © Kitamura et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kitamura, Ryoji
Toyoshima, Takeshi
Tanaka, Hideaki
Kawano, Shintaro
Matsubara, Ryota
Goto, Yuichi
Jinno, Teppei
Maruse, Yasuyuki
Oobu, Kazunari
Nakamura, Seiji
Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma
title Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma
title_full Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma
title_fullStr Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma
title_full_unstemmed Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma
title_short Cytokeratin 17 mRNA as a prognostic marker of oral squamous cell carcinoma
title_sort cytokeratin 17 mrna as a prognostic marker of oral squamous cell carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686526/
https://www.ncbi.nlm.nih.gov/pubmed/29163698
http://dx.doi.org/10.3892/ol.2017.7066
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