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Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions

[Image: see text] Virus-like particles are very interesting tools for application in bionanotechnology, due to their monodisperse features and biocompatibility. In particular, the cowpea chlorotic mottle virus (CCMV) capsid has been studied extensively as it can be assembled and disassembled reversi...

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Autores principales: Schoonen, Lise, Maassen, Sjors, Nolte, Roeland J. M., van Hest, Jan C. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686562/
https://www.ncbi.nlm.nih.gov/pubmed/28631927
http://dx.doi.org/10.1021/acs.biomac.7b00640
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author Schoonen, Lise
Maassen, Sjors
Nolte, Roeland J. M.
van Hest, Jan C. M.
author_facet Schoonen, Lise
Maassen, Sjors
Nolte, Roeland J. M.
van Hest, Jan C. M.
author_sort Schoonen, Lise
collection PubMed
description [Image: see text] Virus-like particles are very interesting tools for application in bionanotechnology, due to their monodisperse features and biocompatibility. In particular, the cowpea chlorotic mottle virus (CCMV) capsid has been studied extensively as it can be assembled and disassembled reversibly, facilitating cargo encapsulation. CCMV is, however, only stable at physiological conditions when its endogenous nucleic acid cargo is present. To gain more flexibility in the type of cargo encapsulated and to broaden the window of operation, it is interesting to improve the stability of the empty virus-like particles. Here, a method is described to utilize the CCMV capsid at close to physiological conditions as a stable, enzyme-filled nanoreactor. As a proof-of-principle, the encapsulation of T4 lysozyme (T4L) was chosen; this enzyme is a promising antibiotic, but its clinical application is hampered by, for example, its cationic character. It was shown that four T4L molecules can successfully be encapsulated inside CCMV capsids, while remaining catalytically active, which could thus improve the enzyme’s application potential.
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spelling pubmed-56865622017-11-16 Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions Schoonen, Lise Maassen, Sjors Nolte, Roeland J. M. van Hest, Jan C. M. Biomacromolecules [Image: see text] Virus-like particles are very interesting tools for application in bionanotechnology, due to their monodisperse features and biocompatibility. In particular, the cowpea chlorotic mottle virus (CCMV) capsid has been studied extensively as it can be assembled and disassembled reversibly, facilitating cargo encapsulation. CCMV is, however, only stable at physiological conditions when its endogenous nucleic acid cargo is present. To gain more flexibility in the type of cargo encapsulated and to broaden the window of operation, it is interesting to improve the stability of the empty virus-like particles. Here, a method is described to utilize the CCMV capsid at close to physiological conditions as a stable, enzyme-filled nanoreactor. As a proof-of-principle, the encapsulation of T4 lysozyme (T4L) was chosen; this enzyme is a promising antibiotic, but its clinical application is hampered by, for example, its cationic character. It was shown that four T4L molecules can successfully be encapsulated inside CCMV capsids, while remaining catalytically active, which could thus improve the enzyme’s application potential. American Chemical Society 2017-06-20 2017-11-13 /pmc/articles/PMC5686562/ /pubmed/28631927 http://dx.doi.org/10.1021/acs.biomac.7b00640 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Schoonen, Lise
Maassen, Sjors
Nolte, Roeland J. M.
van Hest, Jan C. M.
Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions
title Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions
title_full Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions
title_fullStr Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions
title_full_unstemmed Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions
title_short Stabilization of a Virus-Like Particle and Its Application as a Nanoreactor at Physiological Conditions
title_sort stabilization of a virus-like particle and its application as a nanoreactor at physiological conditions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686562/
https://www.ncbi.nlm.nih.gov/pubmed/28631927
http://dx.doi.org/10.1021/acs.biomac.7b00640
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