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Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli
BACKGROUND: Viral protein expression in Escherichia coli (E. coli) is a powerful tool for structural/functional studies as well as for vaccine and diagnostics development. However, numerous factors such as codon bias, mRNA secondary structure and nucleotides distribution, have been indentified to ha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686824/ https://www.ncbi.nlm.nih.gov/pubmed/29137642 http://dx.doi.org/10.1186/s12934-017-0812-8 |