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Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli

BACKGROUND: Viral protein expression in Escherichia coli (E. coli) is a powerful tool for structural/functional studies as well as for vaccine and diagnostics development. However, numerous factors such as codon bias, mRNA secondary structure and nucleotides distribution, have been indentified to ha...

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Detalles Bibliográficos
Autores principales: Behloul, Nouredine, Wei, Wenjuan, Baha, Sarra, Liu, Zhenzhen, Wen, Jiyue, Meng, Jihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686824/
https://www.ncbi.nlm.nih.gov/pubmed/29137642
http://dx.doi.org/10.1186/s12934-017-0812-8