Cargando…

Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model

BACKGROUND: This study aims to investigate the technical feasibility of semi-quantitative and quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the assessment of longitudinal changes of marrow perfusion in a rat osteoporosis model, using bone mineral density (BMD) measur...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Jingqi, Xiong, Zuogang, Zhang, Jiulong, Qiu, Yuyou, Hua, Ting, Tang, Guangyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686959/
https://www.ncbi.nlm.nih.gov/pubmed/29137612
http://dx.doi.org/10.1186/s12891-017-1800-1
_version_ 1783278876613410816
author Zhu, Jingqi
Xiong, Zuogang
Zhang, Jiulong
Qiu, Yuyou
Hua, Ting
Tang, Guangyu
author_facet Zhu, Jingqi
Xiong, Zuogang
Zhang, Jiulong
Qiu, Yuyou
Hua, Ting
Tang, Guangyu
author_sort Zhu, Jingqi
collection PubMed
description BACKGROUND: This study aims to investigate the technical feasibility of semi-quantitative and quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the assessment of longitudinal changes of marrow perfusion in a rat osteoporosis model, using bone mineral density (BMD) measured by micro-computed tomography (micro-CT) and histopathology as the gold standards. METHODS: Fifty rats were randomly assigned to the control group (n=25) and ovariectomy (OVX) group whose bilateral ovaries were excised (n=25). Semi-quantitative and quantitative DCE-MRI, micro-CT, and histopathological examinations were performed on lumbar vertebrae at baseline and 3, 6, 9, and 12 weeks after operation. The differences between the two groups in terms of semi-quantitative DCE-MRI parameter (maximum enhancement, E(max)), quantitative DCE-MRI parameters (volume transfer constant, K(trans); interstitial volume, V(e); and efflux rate constant, K(ep)), micro-CT parameter (BMD), and histopathological parameter (microvessel density, MVD) were compared at each of the time points using an independent-sample t test. The differences in these parameters between baseline and other time points in each group were assessed via Bonferroni’s multiple comparison test. A Pearson correlation analysis was applied to assess the relationships between DCE-MRI, micro-CT, and histopathological parameters. RESULTS: In the OVX group, the E(max) values decreased significantly compared with those of the control group at weeks 6 and 9 (p=0.003 and 0.004, respectively). The K(trans) values decreased significantly compared with those of the control group from week 3 (p<0.05). However, the V(e) values decreased significantly only at week 9 (p=0.032), and no difference in the K(ep) was found between two groups. The BMD values of the OVX group decreased significantly compared with those of the control group from week 3 (p<0.05). Transmission electron microscopy showed tighter gaps between vascular endothelial cells with swollen mitochondria in the OVX group from week 3. The MVD values of the OVX group decreased significantly compared with those of the control group only at week 12 (p=0.023). A weak positive correlation of E(max) and a strong positive correlation of K(trans) with MVD were found. CONCLUSIONS: Compared with semi-quantitative DCE-MRI, the quantitative DCE-MRI parameter K(trans) is a more sensitive and accurate index for detecting early reduced perfusion in osteoporotic bone.
format Online
Article
Text
id pubmed-5686959
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-56869592017-11-21 Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model Zhu, Jingqi Xiong, Zuogang Zhang, Jiulong Qiu, Yuyou Hua, Ting Tang, Guangyu BMC Musculoskelet Disord Research Article BACKGROUND: This study aims to investigate the technical feasibility of semi-quantitative and quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the assessment of longitudinal changes of marrow perfusion in a rat osteoporosis model, using bone mineral density (BMD) measured by micro-computed tomography (micro-CT) and histopathology as the gold standards. METHODS: Fifty rats were randomly assigned to the control group (n=25) and ovariectomy (OVX) group whose bilateral ovaries were excised (n=25). Semi-quantitative and quantitative DCE-MRI, micro-CT, and histopathological examinations were performed on lumbar vertebrae at baseline and 3, 6, 9, and 12 weeks after operation. The differences between the two groups in terms of semi-quantitative DCE-MRI parameter (maximum enhancement, E(max)), quantitative DCE-MRI parameters (volume transfer constant, K(trans); interstitial volume, V(e); and efflux rate constant, K(ep)), micro-CT parameter (BMD), and histopathological parameter (microvessel density, MVD) were compared at each of the time points using an independent-sample t test. The differences in these parameters between baseline and other time points in each group were assessed via Bonferroni’s multiple comparison test. A Pearson correlation analysis was applied to assess the relationships between DCE-MRI, micro-CT, and histopathological parameters. RESULTS: In the OVX group, the E(max) values decreased significantly compared with those of the control group at weeks 6 and 9 (p=0.003 and 0.004, respectively). The K(trans) values decreased significantly compared with those of the control group from week 3 (p<0.05). However, the V(e) values decreased significantly only at week 9 (p=0.032), and no difference in the K(ep) was found between two groups. The BMD values of the OVX group decreased significantly compared with those of the control group from week 3 (p<0.05). Transmission electron microscopy showed tighter gaps between vascular endothelial cells with swollen mitochondria in the OVX group from week 3. The MVD values of the OVX group decreased significantly compared with those of the control group only at week 12 (p=0.023). A weak positive correlation of E(max) and a strong positive correlation of K(trans) with MVD were found. CONCLUSIONS: Compared with semi-quantitative DCE-MRI, the quantitative DCE-MRI parameter K(trans) is a more sensitive and accurate index for detecting early reduced perfusion in osteoporotic bone. BioMed Central 2017-11-14 /pmc/articles/PMC5686959/ /pubmed/29137612 http://dx.doi.org/10.1186/s12891-017-1800-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhu, Jingqi
Xiong, Zuogang
Zhang, Jiulong
Qiu, Yuyou
Hua, Ting
Tang, Guangyu
Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model
title Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model
title_full Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model
title_fullStr Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model
title_full_unstemmed Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model
title_short Comparison of semi-quantitative and quantitative dynamic contrast-enhanced MRI evaluations of vertebral marrow perfusion in a rat osteoporosis model
title_sort comparison of semi-quantitative and quantitative dynamic contrast-enhanced mri evaluations of vertebral marrow perfusion in a rat osteoporosis model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686959/
https://www.ncbi.nlm.nih.gov/pubmed/29137612
http://dx.doi.org/10.1186/s12891-017-1800-1
work_keys_str_mv AT zhujingqi comparisonofsemiquantitativeandquantitativedynamiccontrastenhancedmrievaluationsofvertebralmarrowperfusioninaratosteoporosismodel
AT xiongzuogang comparisonofsemiquantitativeandquantitativedynamiccontrastenhancedmrievaluationsofvertebralmarrowperfusioninaratosteoporosismodel
AT zhangjiulong comparisonofsemiquantitativeandquantitativedynamiccontrastenhancedmrievaluationsofvertebralmarrowperfusioninaratosteoporosismodel
AT qiuyuyou comparisonofsemiquantitativeandquantitativedynamiccontrastenhancedmrievaluationsofvertebralmarrowperfusioninaratosteoporosismodel
AT huating comparisonofsemiquantitativeandquantitativedynamiccontrastenhancedmrievaluationsofvertebralmarrowperfusioninaratosteoporosismodel
AT tangguangyu comparisonofsemiquantitativeandquantitativedynamiccontrastenhancedmrievaluationsofvertebralmarrowperfusioninaratosteoporosismodel