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Differential context-specific impact of individual core promoter elements on transcriptional dynamics

Eukaryotic transcription occurs in bursts that vary in size and frequency, but the contribution of individual core promoter elements to transcriptional bursting is not known. Here we analyze the relative contributions to bursting of the individual core promoter elements—CCAAT, TATAA-like, Sp1BS, and...

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Autores principales: Hendy, Oliver, Campbell, John, Weissman, Jocelyn D., Larson, Daniel R., Singer, Dinah S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687036/
https://www.ncbi.nlm.nih.gov/pubmed/28931597
http://dx.doi.org/10.1091/mbc.E17-06-0408
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author Hendy, Oliver
Campbell, John
Weissman, Jocelyn D.
Larson, Daniel R.
Singer, Dinah S.
author_facet Hendy, Oliver
Campbell, John
Weissman, Jocelyn D.
Larson, Daniel R.
Singer, Dinah S.
author_sort Hendy, Oliver
collection PubMed
description Eukaryotic transcription occurs in bursts that vary in size and frequency, but the contribution of individual core promoter elements to transcriptional bursting is not known. Here we analyze the relative contributions to bursting of the individual core promoter elements—CCAAT, TATAA-like, Sp1BS, and Inr—of an MHC class I gene in primary B-cells during both basal and activated transcription. The TATAA-like, Sp1BS, and Inr elements all function as negative regulators of transcription, and each was found to contribute differentially to the overall bursting pattern of the promoter during basal transcription. Whereas the Sp1BS element regulates burst size, the Inr element regulates burst frequency. The TATAA-like element contributes to both. Surprisingly, each element has a distinct role in bursting during transcriptional activation by γ-interferon. The CCAAT element does not contribute significantly to the constitutive transcriptional dynamics of primary B-cells, but modulates both burst size and frequency in response to γ-interferon activation. The ability of core promoter elements to modulate transcriptional bursting individually allows combinatorial fine-tuning of the level of MHC class I gene expression in response to intrinsic and extrinsic signals.
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spelling pubmed-56870362018-01-22 Differential context-specific impact of individual core promoter elements on transcriptional dynamics Hendy, Oliver Campbell, John Weissman, Jocelyn D. Larson, Daniel R. Singer, Dinah S. Mol Biol Cell Articles Eukaryotic transcription occurs in bursts that vary in size and frequency, but the contribution of individual core promoter elements to transcriptional bursting is not known. Here we analyze the relative contributions to bursting of the individual core promoter elements—CCAAT, TATAA-like, Sp1BS, and Inr—of an MHC class I gene in primary B-cells during both basal and activated transcription. The TATAA-like, Sp1BS, and Inr elements all function as negative regulators of transcription, and each was found to contribute differentially to the overall bursting pattern of the promoter during basal transcription. Whereas the Sp1BS element regulates burst size, the Inr element regulates burst frequency. The TATAA-like element contributes to both. Surprisingly, each element has a distinct role in bursting during transcriptional activation by γ-interferon. The CCAAT element does not contribute significantly to the constitutive transcriptional dynamics of primary B-cells, but modulates both burst size and frequency in response to γ-interferon activation. The ability of core promoter elements to modulate transcriptional bursting individually allows combinatorial fine-tuning of the level of MHC class I gene expression in response to intrinsic and extrinsic signals. The American Society for Cell Biology 2017-11-07 /pmc/articles/PMC5687036/ /pubmed/28931597 http://dx.doi.org/10.1091/mbc.E17-06-0408 Text en © 2017 Hendy et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Hendy, Oliver
Campbell, John
Weissman, Jocelyn D.
Larson, Daniel R.
Singer, Dinah S.
Differential context-specific impact of individual core promoter elements on transcriptional dynamics
title Differential context-specific impact of individual core promoter elements on transcriptional dynamics
title_full Differential context-specific impact of individual core promoter elements on transcriptional dynamics
title_fullStr Differential context-specific impact of individual core promoter elements on transcriptional dynamics
title_full_unstemmed Differential context-specific impact of individual core promoter elements on transcriptional dynamics
title_short Differential context-specific impact of individual core promoter elements on transcriptional dynamics
title_sort differential context-specific impact of individual core promoter elements on transcriptional dynamics
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687036/
https://www.ncbi.nlm.nih.gov/pubmed/28931597
http://dx.doi.org/10.1091/mbc.E17-06-0408
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