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Testing the time-of-flight model for flagellar length sensing

Cilia and flagella are microtubule-based organelles that protrude from the surface of most cells, are important to the sensing of extracellular signals, and make a driving force for fluid flow. Maintenance of flagellar length requires an active transport process known as intraflagellar transport (IF...

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Autores principales: Ishikawa, Hiroaki, Marshall, Wallace F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687043/
https://www.ncbi.nlm.nih.gov/pubmed/28931591
http://dx.doi.org/10.1091/mbc.E17-06-0384
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author Ishikawa, Hiroaki
Marshall, Wallace F.
author_facet Ishikawa, Hiroaki
Marshall, Wallace F.
author_sort Ishikawa, Hiroaki
collection PubMed
description Cilia and flagella are microtubule-based organelles that protrude from the surface of most cells, are important to the sensing of extracellular signals, and make a driving force for fluid flow. Maintenance of flagellar length requires an active transport process known as intraflagellar transport (IFT). Recent studies reveal that the amount of IFT injection negatively correlates with the length of flagella. These observations suggest that a length-dependent feedback regulates IFT. However, it is unknown how cells recognize the length of flagella and control IFT. Several theoretical models try to explain this feedback system. We focused on one of the models, the “time-of-flight” model, which measures the length of flagella on the basis of the travel time of IFT protein in the flagellar compartment. We tested the time-of-flight model using Chlamydomonas dynein mutant cells, which show slower retrograde transport speed. The amount of IFT injection in dynein mutant cells was higher than that in control cells. This observation does not support the prediction of the time-of-flight model and suggests that Chlamydomonas uses another length-control feedback system rather than that described by the time-of-flight model.
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spelling pubmed-56870432018-01-22 Testing the time-of-flight model for flagellar length sensing Ishikawa, Hiroaki Marshall, Wallace F. Mol Biol Cell Articles Cilia and flagella are microtubule-based organelles that protrude from the surface of most cells, are important to the sensing of extracellular signals, and make a driving force for fluid flow. Maintenance of flagellar length requires an active transport process known as intraflagellar transport (IFT). Recent studies reveal that the amount of IFT injection negatively correlates with the length of flagella. These observations suggest that a length-dependent feedback regulates IFT. However, it is unknown how cells recognize the length of flagella and control IFT. Several theoretical models try to explain this feedback system. We focused on one of the models, the “time-of-flight” model, which measures the length of flagella on the basis of the travel time of IFT protein in the flagellar compartment. We tested the time-of-flight model using Chlamydomonas dynein mutant cells, which show slower retrograde transport speed. The amount of IFT injection in dynein mutant cells was higher than that in control cells. This observation does not support the prediction of the time-of-flight model and suggests that Chlamydomonas uses another length-control feedback system rather than that described by the time-of-flight model. The American Society for Cell Biology 2017-11-07 /pmc/articles/PMC5687043/ /pubmed/28931591 http://dx.doi.org/10.1091/mbc.E17-06-0384 Text en © 2017 Ishikawa and Marshall. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Ishikawa, Hiroaki
Marshall, Wallace F.
Testing the time-of-flight model for flagellar length sensing
title Testing the time-of-flight model for flagellar length sensing
title_full Testing the time-of-flight model for flagellar length sensing
title_fullStr Testing the time-of-flight model for flagellar length sensing
title_full_unstemmed Testing the time-of-flight model for flagellar length sensing
title_short Testing the time-of-flight model for flagellar length sensing
title_sort testing the time-of-flight model for flagellar length sensing
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687043/
https://www.ncbi.nlm.nih.gov/pubmed/28931591
http://dx.doi.org/10.1091/mbc.E17-06-0384
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