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Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness
KEY MESSAGE: This work adds a new player, HER2, downstream of the perception of E-2-hexenal, a green leaf volatile, and shows that E-2-hexenal specifically changes the redox status of the mitochondria. ABSTRACT: It is widely accepted that plants produce and respond to green leaf volatiles (GLVs), bu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688203/ https://www.ncbi.nlm.nih.gov/pubmed/28918565 http://dx.doi.org/10.1007/s11103-017-0659-8 |
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author | Scala, Alessandra Mirabella, Rossana Goedhart, Joachim de Vries, Michel Haring, Michel A. Schuurink, Robert C. |
author_facet | Scala, Alessandra Mirabella, Rossana Goedhart, Joachim de Vries, Michel Haring, Michel A. Schuurink, Robert C. |
author_sort | Scala, Alessandra |
collection | PubMed |
description | KEY MESSAGE: This work adds a new player, HER2, downstream of the perception of E-2-hexenal, a green leaf volatile, and shows that E-2-hexenal specifically changes the redox status of the mitochondria. ABSTRACT: It is widely accepted that plants produce and respond to green leaf volatiles (GLVs), but the molecular components involved in transducing their perception are largely unknown. The GLV E-2-hexenal inhibits root elongation in seedlings and, using this phenotype, we isolated E-2-hexenal response (her) Arabidopsis thaliana mutants. Using map-based cloning we positioned the her2 mutation to the At5g63620 locus, resulting in a phenylalanine instead of serine on position 223. Knockdown and overexpression lines of HER2 confirmed the role of HER2, which encodes an oxidoreductase, in the responsiveness to E-2-hexenal. Since E-2-hexenal is a reactive electrophile species, which are known to influence the redox status of cells, we utilized redox sensitive GFP2 (roGFP2) to determine the redox status of E-2-hexenal-treated root cells. Since the signal peptide of HER2 directed mCherry to the mitochondria, we targeted the expression of roGFP2 to this organelle besides the cytosol. E-2-hexenal specifically induced a change in the redox status in the mitochondria. We did not see a difference in the redox status in her2 compared to wild-type Arabidopsis. Still, the mitochondrial redox status did not change with Z-3-hexenol, another abundant GLV. These results indicate that HER2 is involved in transducing the perception of E-2-hexenal, which changes the redox status of the mitochondria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-017-0659-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5688203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-56882032017-11-30 Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness Scala, Alessandra Mirabella, Rossana Goedhart, Joachim de Vries, Michel Haring, Michel A. Schuurink, Robert C. Plant Mol Biol Article KEY MESSAGE: This work adds a new player, HER2, downstream of the perception of E-2-hexenal, a green leaf volatile, and shows that E-2-hexenal specifically changes the redox status of the mitochondria. ABSTRACT: It is widely accepted that plants produce and respond to green leaf volatiles (GLVs), but the molecular components involved in transducing their perception are largely unknown. The GLV E-2-hexenal inhibits root elongation in seedlings and, using this phenotype, we isolated E-2-hexenal response (her) Arabidopsis thaliana mutants. Using map-based cloning we positioned the her2 mutation to the At5g63620 locus, resulting in a phenylalanine instead of serine on position 223. Knockdown and overexpression lines of HER2 confirmed the role of HER2, which encodes an oxidoreductase, in the responsiveness to E-2-hexenal. Since E-2-hexenal is a reactive electrophile species, which are known to influence the redox status of cells, we utilized redox sensitive GFP2 (roGFP2) to determine the redox status of E-2-hexenal-treated root cells. Since the signal peptide of HER2 directed mCherry to the mitochondria, we targeted the expression of roGFP2 to this organelle besides the cytosol. E-2-hexenal specifically induced a change in the redox status in the mitochondria. We did not see a difference in the redox status in her2 compared to wild-type Arabidopsis. Still, the mitochondrial redox status did not change with Z-3-hexenol, another abundant GLV. These results indicate that HER2 is involved in transducing the perception of E-2-hexenal, which changes the redox status of the mitochondria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-017-0659-8) contains supplementary material, which is available to authorized users. Springer Netherlands 2017-09-16 2017 /pmc/articles/PMC5688203/ /pubmed/28918565 http://dx.doi.org/10.1007/s11103-017-0659-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Scala, Alessandra Mirabella, Rossana Goedhart, Joachim de Vries, Michel Haring, Michel A. Schuurink, Robert C. Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness |
title | Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness |
title_full | Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness |
title_fullStr | Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness |
title_full_unstemmed | Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness |
title_short | Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness |
title_sort | forward genetic screens identify a role for the mitochondrial her2 in e-2-hexenal responsiveness |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688203/ https://www.ncbi.nlm.nih.gov/pubmed/28918565 http://dx.doi.org/10.1007/s11103-017-0659-8 |
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