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Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi

Rhodococcus equi (R. equi) is an intracellular macrophage-tropic pathogen with potential for causing fatal pyogranulomatous pneumonia in foals between 1 and 6 months of age. In this study, we sought to determine whether infection of macrophages with R. equi could lead to the induction of autophagy....

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Autores principales: Chandramani-Shivalingappa, Prashanth, Bhandari, Mahesh, Wiechert, Sarah A., Gilbertie, Jessica, Jones, Douglas E., Sponseller, Brett A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688232/
https://www.ncbi.nlm.nih.gov/pubmed/29230347
http://dx.doi.org/10.1155/2017/8135737
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author Chandramani-Shivalingappa, Prashanth
Bhandari, Mahesh
Wiechert, Sarah A.
Gilbertie, Jessica
Jones, Douglas E.
Sponseller, Brett A.
author_facet Chandramani-Shivalingappa, Prashanth
Bhandari, Mahesh
Wiechert, Sarah A.
Gilbertie, Jessica
Jones, Douglas E.
Sponseller, Brett A.
author_sort Chandramani-Shivalingappa, Prashanth
collection PubMed
description Rhodococcus equi (R. equi) is an intracellular macrophage-tropic pathogen with potential for causing fatal pyogranulomatous pneumonia in foals between 1 and 6 months of age. In this study, we sought to determine whether infection of macrophages with R. equi could lead to the induction of autophagy. Murine bone marrow derived macrophages (BMDM) were infected with R. equi for various time intervals and analyzed for upregulation of autophagy proteins and accumulation of autophagosomes relative to uninfected controls. Western blot analysis showed a progressive increase in LC3-II and Beclin1 levels in a time-dependent manner. The functional accumulation of autophagosomes detected with monodansylcadaverine further supported the enhanced induction of autophagy in BMDM infected with R. equi. In addition, infection of BMDM with R. equi induced generation of reactive oxygen species (ROS) in a time-dependent manner. These data are consistent with reports documenting the role of ROS in induction of autophagy and indicate that the infection of macrophages by R. equi elicits innate host defense mechanisms.
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spelling pubmed-56882322017-12-11 Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi Chandramani-Shivalingappa, Prashanth Bhandari, Mahesh Wiechert, Sarah A. Gilbertie, Jessica Jones, Douglas E. Sponseller, Brett A. Scientifica (Cairo) Research Article Rhodococcus equi (R. equi) is an intracellular macrophage-tropic pathogen with potential for causing fatal pyogranulomatous pneumonia in foals between 1 and 6 months of age. In this study, we sought to determine whether infection of macrophages with R. equi could lead to the induction of autophagy. Murine bone marrow derived macrophages (BMDM) were infected with R. equi for various time intervals and analyzed for upregulation of autophagy proteins and accumulation of autophagosomes relative to uninfected controls. Western blot analysis showed a progressive increase in LC3-II and Beclin1 levels in a time-dependent manner. The functional accumulation of autophagosomes detected with monodansylcadaverine further supported the enhanced induction of autophagy in BMDM infected with R. equi. In addition, infection of BMDM with R. equi induced generation of reactive oxygen species (ROS) in a time-dependent manner. These data are consistent with reports documenting the role of ROS in induction of autophagy and indicate that the infection of macrophages by R. equi elicits innate host defense mechanisms. Hindawi 2017 2017-11-01 /pmc/articles/PMC5688232/ /pubmed/29230347 http://dx.doi.org/10.1155/2017/8135737 Text en Copyright © 2017 Prashanth Chandramani-Shivalingappa et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chandramani-Shivalingappa, Prashanth
Bhandari, Mahesh
Wiechert, Sarah A.
Gilbertie, Jessica
Jones, Douglas E.
Sponseller, Brett A.
Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi
title Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi
title_full Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi
title_fullStr Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi
title_full_unstemmed Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi
title_short Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi
title_sort induction of reactive intermediates and autophagy-related proteins upon infection of macrophages with rhodococcus equi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688232/
https://www.ncbi.nlm.nih.gov/pubmed/29230347
http://dx.doi.org/10.1155/2017/8135737
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