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Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury
OBJECTIVE: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism. METHODS: After designing and synthesizing three pairs of siRNA that ef...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kare Publishing
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689047/ https://www.ncbi.nlm.nih.gov/pubmed/28639948 http://dx.doi.org/10.14744/AnatolJCardiol.2017.7763 |
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author | Gong, Ge Li, Yanyan Yang, Xinxing Geng, Hongyu Lu, Xinzheng Wang, Liansheng Yang, Zhijian |
author_facet | Gong, Ge Li, Yanyan Yang, Xinxing Geng, Hongyu Lu, Xinzheng Wang, Liansheng Yang, Zhijian |
author_sort | Gong, Ge |
collection | PubMed |
description | OBJECTIVE: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism. METHODS: After designing and synthesizing three pairs of siRNA that effectively reduced IL28RA gene expression in vitro (siRNA-6158, siRNA-6160, and siRNA-6162), primary neonatal rat cardiomyocytes were transfected using a liposome transfection method. Six groups were included based on the siRNA that was used and the treatment simulating reperfusion injury: control group, H/R group, H/R+negative control group, H/R+siRNA-6158 group, H/R+siRNA-6160 group, and H/R+siRNA-6162 group. Cell survival and apoptosis rates were measured along with lactate dehydrogenase levels in the cell culture supernatant. Protein levels of IL28RA, phosphatidylinositol 3-kinase, catalytic subunit gamma (PI3KCG), Bcl-2, Bax, and b-actin were also measured. RESULTS: The H/R+siRNA-6158 and H/R+siRNA-6160 groups had significantly higher survival rates and increased PI3KCG-to-b-actin and Bcl-2-to-Bax ratios than the the H/R and H/R+negative control groups (p<0.05). The H/R+siRNA-6158 and H/R+siRNA-6160 groups also exhibited reduced rates of apoptosis and reduced IL28RA-to-b-actin ratios (p<0.05). No significant difference was observed among the H/R+siRNA-6162, H/R, and H/R+negative control groups. CONCLUSION: IL28RA siRNA-6158 and -6160 were able to protect cardiomyocytes from H/R injury by inhibiting apoptosis. This strategy of inhibiting IL28RA gene expression may reduce reperfusion injury in the treatment of patients with acute myocardial infarction. |
format | Online Article Text |
id | pubmed-5689047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Kare Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-56890472017-11-21 Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury Gong, Ge Li, Yanyan Yang, Xinxing Geng, Hongyu Lu, Xinzheng Wang, Liansheng Yang, Zhijian Anatol J Cardiol Original Investigation OBJECTIVE: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism. METHODS: After designing and synthesizing three pairs of siRNA that effectively reduced IL28RA gene expression in vitro (siRNA-6158, siRNA-6160, and siRNA-6162), primary neonatal rat cardiomyocytes were transfected using a liposome transfection method. Six groups were included based on the siRNA that was used and the treatment simulating reperfusion injury: control group, H/R group, H/R+negative control group, H/R+siRNA-6158 group, H/R+siRNA-6160 group, and H/R+siRNA-6162 group. Cell survival and apoptosis rates were measured along with lactate dehydrogenase levels in the cell culture supernatant. Protein levels of IL28RA, phosphatidylinositol 3-kinase, catalytic subunit gamma (PI3KCG), Bcl-2, Bax, and b-actin were also measured. RESULTS: The H/R+siRNA-6158 and H/R+siRNA-6160 groups had significantly higher survival rates and increased PI3KCG-to-b-actin and Bcl-2-to-Bax ratios than the the H/R and H/R+negative control groups (p<0.05). The H/R+siRNA-6158 and H/R+siRNA-6160 groups also exhibited reduced rates of apoptosis and reduced IL28RA-to-b-actin ratios (p<0.05). No significant difference was observed among the H/R+siRNA-6162, H/R, and H/R+negative control groups. CONCLUSION: IL28RA siRNA-6158 and -6160 were able to protect cardiomyocytes from H/R injury by inhibiting apoptosis. This strategy of inhibiting IL28RA gene expression may reduce reperfusion injury in the treatment of patients with acute myocardial infarction. Kare Publishing 2017-09 2017-06-21 /pmc/articles/PMC5689047/ /pubmed/28639948 http://dx.doi.org/10.14744/AnatolJCardiol.2017.7763 Text en Copyright: © 2017 Turkish Society of Cardiology http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License |
spellingShingle | Original Investigation Gong, Ge Li, Yanyan Yang, Xinxing Geng, Hongyu Lu, Xinzheng Wang, Liansheng Yang, Zhijian Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury |
title | Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury |
title_full | Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury |
title_fullStr | Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury |
title_full_unstemmed | Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury |
title_short | Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury |
title_sort | protective effects of il28ra sirna on cardiomyocytes in hypoxia/reoxygenation injury |
topic | Original Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689047/ https://www.ncbi.nlm.nih.gov/pubmed/28639948 http://dx.doi.org/10.14744/AnatolJCardiol.2017.7763 |
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