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Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury

OBJECTIVE: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism. METHODS: After designing and synthesizing three pairs of siRNA that ef...

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Autores principales: Gong, Ge, Li, Yanyan, Yang, Xinxing, Geng, Hongyu, Lu, Xinzheng, Wang, Liansheng, Yang, Zhijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kare Publishing 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689047/
https://www.ncbi.nlm.nih.gov/pubmed/28639948
http://dx.doi.org/10.14744/AnatolJCardiol.2017.7763
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author Gong, Ge
Li, Yanyan
Yang, Xinxing
Geng, Hongyu
Lu, Xinzheng
Wang, Liansheng
Yang, Zhijian
author_facet Gong, Ge
Li, Yanyan
Yang, Xinxing
Geng, Hongyu
Lu, Xinzheng
Wang, Liansheng
Yang, Zhijian
author_sort Gong, Ge
collection PubMed
description OBJECTIVE: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism. METHODS: After designing and synthesizing three pairs of siRNA that effectively reduced IL28RA gene expression in vitro (siRNA-6158, siRNA-6160, and siRNA-6162), primary neonatal rat cardiomyocytes were transfected using a liposome transfection method. Six groups were included based on the siRNA that was used and the treatment simulating reperfusion injury: control group, H/R group, H/R+negative control group, H/R+siRNA-6158 group, H/R+siRNA-6160 group, and H/R+siRNA-6162 group. Cell survival and apoptosis rates were measured along with lactate dehydrogenase levels in the cell culture supernatant. Protein levels of IL28RA, phosphatidylinositol 3-kinase, catalytic subunit gamma (PI3KCG), Bcl-2, Bax, and b-actin were also measured. RESULTS: The H/R+siRNA-6158 and H/R+siRNA-6160 groups had significantly higher survival rates and increased PI3KCG-to-b-actin and Bcl-2-to-Bax ratios than the the H/R and H/R+negative control groups (p<0.05). The H/R+siRNA-6158 and H/R+siRNA-6160 groups also exhibited reduced rates of apoptosis and reduced IL28RA-to-b-actin ratios (p<0.05). No significant difference was observed among the H/R+siRNA-6162, H/R, and H/R+negative control groups. CONCLUSION: IL28RA siRNA-6158 and -6160 were able to protect cardiomyocytes from H/R injury by inhibiting apoptosis. This strategy of inhibiting IL28RA gene expression may reduce reperfusion injury in the treatment of patients with acute myocardial infarction.
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spelling pubmed-56890472017-11-21 Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury Gong, Ge Li, Yanyan Yang, Xinxing Geng, Hongyu Lu, Xinzheng Wang, Liansheng Yang, Zhijian Anatol J Cardiol Original Investigation OBJECTIVE: We demonstrate the protective effects of the siRNA-mediated inhibition of the interleukin-28 receptor alpha (IL28RA) subunit on cardiomyocytes in hypoxia/reoxygenation (H/R) injury and explore the associated mechanism. METHODS: After designing and synthesizing three pairs of siRNA that effectively reduced IL28RA gene expression in vitro (siRNA-6158, siRNA-6160, and siRNA-6162), primary neonatal rat cardiomyocytes were transfected using a liposome transfection method. Six groups were included based on the siRNA that was used and the treatment simulating reperfusion injury: control group, H/R group, H/R+negative control group, H/R+siRNA-6158 group, H/R+siRNA-6160 group, and H/R+siRNA-6162 group. Cell survival and apoptosis rates were measured along with lactate dehydrogenase levels in the cell culture supernatant. Protein levels of IL28RA, phosphatidylinositol 3-kinase, catalytic subunit gamma (PI3KCG), Bcl-2, Bax, and b-actin were also measured. RESULTS: The H/R+siRNA-6158 and H/R+siRNA-6160 groups had significantly higher survival rates and increased PI3KCG-to-b-actin and Bcl-2-to-Bax ratios than the the H/R and H/R+negative control groups (p<0.05). The H/R+siRNA-6158 and H/R+siRNA-6160 groups also exhibited reduced rates of apoptosis and reduced IL28RA-to-b-actin ratios (p<0.05). No significant difference was observed among the H/R+siRNA-6162, H/R, and H/R+negative control groups. CONCLUSION: IL28RA siRNA-6158 and -6160 were able to protect cardiomyocytes from H/R injury by inhibiting apoptosis. This strategy of inhibiting IL28RA gene expression may reduce reperfusion injury in the treatment of patients with acute myocardial infarction. Kare Publishing 2017-09 2017-06-21 /pmc/articles/PMC5689047/ /pubmed/28639948 http://dx.doi.org/10.14744/AnatolJCardiol.2017.7763 Text en Copyright: © 2017 Turkish Society of Cardiology http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Original Investigation
Gong, Ge
Li, Yanyan
Yang, Xinxing
Geng, Hongyu
Lu, Xinzheng
Wang, Liansheng
Yang, Zhijian
Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury
title Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury
title_full Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury
title_fullStr Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury
title_full_unstemmed Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury
title_short Protective effects of IL28RA siRNA on cardiomyocytes in hypoxia/reoxygenation injury
title_sort protective effects of il28ra sirna on cardiomyocytes in hypoxia/reoxygenation injury
topic Original Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689047/
https://www.ncbi.nlm.nih.gov/pubmed/28639948
http://dx.doi.org/10.14744/AnatolJCardiol.2017.7763
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