Cargando…

Lethality of inappropriate plasma exposure on chicken embryonic development

In this study, we examined the effects of non-thermal dielectric barrier discharge plasma on embryonic development in chicken eggs in order to determine the optimal level of plasma exposure for the promotion of embryonic growth. We exposed developing chicken embryos at either Hamburger-Hamilton (HH)...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Jiao Jiao, Jo, Jin Oh, Huynh, Do Luong, Ghosh, Mrinmoy, Kim, Nameun, Lee, Sang Baek, Lee, Hak Kyo, Mok, Young Sun, Kwon, Taeho, Jeong, Dong Kee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689637/
https://www.ncbi.nlm.nih.gov/pubmed/29156747
http://dx.doi.org/10.18632/oncotarget.21105
_version_ 1783279423300042752
author Zhang, Jiao Jiao
Jo, Jin Oh
Huynh, Do Luong
Ghosh, Mrinmoy
Kim, Nameun
Lee, Sang Baek
Lee, Hak Kyo
Mok, Young Sun
Kwon, Taeho
Jeong, Dong Kee
author_facet Zhang, Jiao Jiao
Jo, Jin Oh
Huynh, Do Luong
Ghosh, Mrinmoy
Kim, Nameun
Lee, Sang Baek
Lee, Hak Kyo
Mok, Young Sun
Kwon, Taeho
Jeong, Dong Kee
author_sort Zhang, Jiao Jiao
collection PubMed
description In this study, we examined the effects of non-thermal dielectric barrier discharge plasma on embryonic development in chicken eggs in order to determine the optimal level of plasma exposure for the promotion of embryonic growth. We exposed developing chicken embryos at either Hamburger-Hamilton (HH) stage 04 or HH 20 to plasma at voltages of 11.7 kV to 27.6 kV. Our results show exposure at 11.7 kV for 1 min promoted chicken embryonic development, but exposure to more duration and intensity of plasma resulted in dose-dependent embryonic death and HH 20 stage embryos survive longer than those at stage HH 04. Furthermore, plasma exposure for 4 min increased the production of reactive oxygen species (ROS) and inactivated the nuclear factor erythroid 2-related factor 2 (NRF2)-antioxidant response signaling pathway, resulting in suppression of antioxidant enzymes in the skeletal muscle tissue of the dead embryos. We also found decreased levels of adenosine triphosphate production and reductions in the expression levels of several growth-related genes and proteins. These findings indicate that inappropriate plasma exposure causes dose-dependent embryonic death via excessive accumulation of ROS, NRF2-antioxidant signaling pathway disruption, and decreased growth factor expression.
format Online
Article
Text
id pubmed-5689637
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-56896372017-11-17 Lethality of inappropriate plasma exposure on chicken embryonic development Zhang, Jiao Jiao Jo, Jin Oh Huynh, Do Luong Ghosh, Mrinmoy Kim, Nameun Lee, Sang Baek Lee, Hak Kyo Mok, Young Sun Kwon, Taeho Jeong, Dong Kee Oncotarget Research Paper In this study, we examined the effects of non-thermal dielectric barrier discharge plasma on embryonic development in chicken eggs in order to determine the optimal level of plasma exposure for the promotion of embryonic growth. We exposed developing chicken embryos at either Hamburger-Hamilton (HH) stage 04 or HH 20 to plasma at voltages of 11.7 kV to 27.6 kV. Our results show exposure at 11.7 kV for 1 min promoted chicken embryonic development, but exposure to more duration and intensity of plasma resulted in dose-dependent embryonic death and HH 20 stage embryos survive longer than those at stage HH 04. Furthermore, plasma exposure for 4 min increased the production of reactive oxygen species (ROS) and inactivated the nuclear factor erythroid 2-related factor 2 (NRF2)-antioxidant response signaling pathway, resulting in suppression of antioxidant enzymes in the skeletal muscle tissue of the dead embryos. We also found decreased levels of adenosine triphosphate production and reductions in the expression levels of several growth-related genes and proteins. These findings indicate that inappropriate plasma exposure causes dose-dependent embryonic death via excessive accumulation of ROS, NRF2-antioxidant signaling pathway disruption, and decreased growth factor expression. Impact Journals LLC 2017-09-20 /pmc/articles/PMC5689637/ /pubmed/29156747 http://dx.doi.org/10.18632/oncotarget.21105 Text en Copyright: © 2017 Zhang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhang, Jiao Jiao
Jo, Jin Oh
Huynh, Do Luong
Ghosh, Mrinmoy
Kim, Nameun
Lee, Sang Baek
Lee, Hak Kyo
Mok, Young Sun
Kwon, Taeho
Jeong, Dong Kee
Lethality of inappropriate plasma exposure on chicken embryonic development
title Lethality of inappropriate plasma exposure on chicken embryonic development
title_full Lethality of inappropriate plasma exposure on chicken embryonic development
title_fullStr Lethality of inappropriate plasma exposure on chicken embryonic development
title_full_unstemmed Lethality of inappropriate plasma exposure on chicken embryonic development
title_short Lethality of inappropriate plasma exposure on chicken embryonic development
title_sort lethality of inappropriate plasma exposure on chicken embryonic development
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689637/
https://www.ncbi.nlm.nih.gov/pubmed/29156747
http://dx.doi.org/10.18632/oncotarget.21105
work_keys_str_mv AT zhangjiaojiao lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT jojinoh lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT huynhdoluong lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT ghoshmrinmoy lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT kimnameun lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT leesangbaek lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT leehakkyo lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT mokyoungsun lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT kwontaeho lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment
AT jeongdongkee lethalityofinappropriateplasmaexposureonchickenembryonicdevelopment