Cargando…
Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model
The unfavourable clinical outcomes of host cell-seeded scaffolds for bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhan...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689699/ https://www.ncbi.nlm.nih.gov/pubmed/29156809 http://dx.doi.org/10.18632/oncotarget.21211 |
_version_ | 1783279438442528768 |
---|---|
author | Xiao, Dongdong Wang, Qiong Yan, Hao Lv, Xiangguo Zhao, Yang Zhou, Zhe Zhang, Ming Sun, Qian Sun, Kang Li, Wei Lu, Mujun |
author_facet | Xiao, Dongdong Wang, Qiong Yan, Hao Lv, Xiangguo Zhao, Yang Zhou, Zhe Zhang, Ming Sun, Qian Sun, Kang Li, Wei Lu, Mujun |
author_sort | Xiao, Dongdong |
collection | PubMed |
description | The unfavourable clinical outcomes of host cell-seeded scaffolds for bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing bladder reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation. |
format | Online Article Text |
id | pubmed-5689699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-56896992017-11-17 Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model Xiao, Dongdong Wang, Qiong Yan, Hao Lv, Xiangguo Zhao, Yang Zhou, Zhe Zhang, Ming Sun, Qian Sun, Kang Li, Wei Lu, Mujun Oncotarget Research Paper The unfavourable clinical outcomes of host cell-seeded scaffolds for bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing bladder reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation. Impact Journals LLC 2017-09-23 /pmc/articles/PMC5689699/ /pubmed/29156809 http://dx.doi.org/10.18632/oncotarget.21211 Text en Copyright: © 2017 Xiao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xiao, Dongdong Wang, Qiong Yan, Hao Lv, Xiangguo Zhao, Yang Zhou, Zhe Zhang, Ming Sun, Qian Sun, Kang Li, Wei Lu, Mujun Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model |
title | Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model |
title_full | Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model |
title_fullStr | Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model |
title_full_unstemmed | Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model |
title_short | Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model |
title_sort | adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689699/ https://www.ncbi.nlm.nih.gov/pubmed/29156809 http://dx.doi.org/10.18632/oncotarget.21211 |
work_keys_str_mv | AT xiaodongdong adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT wangqiong adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT yanhao adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT lvxiangguo adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT zhaoyang adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT zhouzhe adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT zhangming adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT sunqian adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT sunkang adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT liwei adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel AT lumujun adiposederivedstemcellsseededbladderacellularmatrixgraftsilkfibroinenhancesbladderreconstructioninaratmodel |