A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways

Here, the immunomodulatory effects of water-soluble polysaccharide from Grifola frondosa on RAW264.7 macrophages and its molecular mechanisms were investigated. G. frondosa polysaccharide could obviously enhance immunostimulatory activity such as the release of nitric oxide and cytokine production....

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Detalles Bibliográficos
Autores principales: Hou, Lihua, Meng, Meng, Chen, Yuanyuan, Wang, Chunling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689710/
https://www.ncbi.nlm.nih.gov/pubmed/29156820
http://dx.doi.org/10.18632/oncotarget.21252
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author Hou, Lihua
Meng, Meng
Chen, Yuanyuan
Wang, Chunling
author_facet Hou, Lihua
Meng, Meng
Chen, Yuanyuan
Wang, Chunling
author_sort Hou, Lihua
collection PubMed
description Here, the immunomodulatory effects of water-soluble polysaccharide from Grifola frondosa on RAW264.7 macrophages and its molecular mechanisms were investigated. G. frondosa polysaccharide could obviously enhance immunostimulatory activity such as the release of nitric oxide and cytokine production. Western blotting results showed that G. frondosa polysaccharide elevated the TLR4, which might act as an upstream regulator of MyD88 induced G. frondosa polysaccharide. MyD88 promoted IKKβ in endochylema and translocate NF-κB p65 subunit into the nucleus which increased the NO production and cytokine/chemokines level. The results suggested that G. frondosa polysaccharide activated macrophages through TLR4-MyD88-IKKβ-NF-κBp65 signaling pathways.
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spelling pubmed-56897102017-11-17 A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways Hou, Lihua Meng, Meng Chen, Yuanyuan Wang, Chunling Oncotarget Research Paper Here, the immunomodulatory effects of water-soluble polysaccharide from Grifola frondosa on RAW264.7 macrophages and its molecular mechanisms were investigated. G. frondosa polysaccharide could obviously enhance immunostimulatory activity such as the release of nitric oxide and cytokine production. Western blotting results showed that G. frondosa polysaccharide elevated the TLR4, which might act as an upstream regulator of MyD88 induced G. frondosa polysaccharide. MyD88 promoted IKKβ in endochylema and translocate NF-κB p65 subunit into the nucleus which increased the NO production and cytokine/chemokines level. The results suggested that G. frondosa polysaccharide activated macrophages through TLR4-MyD88-IKKβ-NF-κBp65 signaling pathways. Impact Journals LLC 2017-09-23 /pmc/articles/PMC5689710/ /pubmed/29156820 http://dx.doi.org/10.18632/oncotarget.21252 Text en Copyright: © 2017 Hou et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Hou, Lihua
Meng, Meng
Chen, Yuanyuan
Wang, Chunling
A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways
title A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways
title_full A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways
title_fullStr A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways
title_full_unstemmed A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways
title_short A water-soluble polysaccharide from Grifola frondosa induced macrophages activation via TLR4-MyD88-IKKβ-NF-κB p65 pathways
title_sort water-soluble polysaccharide from grifola frondosa induced macrophages activation via tlr4-myd88-ikkβ-nf-κb p65 pathways
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689710/
https://www.ncbi.nlm.nih.gov/pubmed/29156820
http://dx.doi.org/10.18632/oncotarget.21252
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