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Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID

General transcription factor TFIID is a key component of RNA polymerase II transcription initiation. Human TFIID is a megadalton-sized complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core promoter DNA, recognizing the TATA-box. We identified a ternary...

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Autores principales: Gupta, Kapil, Watson, Aleksandra A, Baptista, Tiago, Scheer, Elisabeth, Chambers, Anna L, Koehler, Christine, Zou, Juan, Obong-Ebong, Ima, Kandiah, Eaazhisai, Temblador, Arturo, Round, Adam, Forest, Eric, Man, Petr, Bieniossek, Christoph, Laue, Ernest D, Lemke, Edward A, Rappsilber, Juri, Robinson, Carol V, Devys, Didier, Tora, Làszlò, Berger, Imre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690282/
https://www.ncbi.nlm.nih.gov/pubmed/29111974
http://dx.doi.org/10.7554/eLife.30395
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author Gupta, Kapil
Watson, Aleksandra A
Baptista, Tiago
Scheer, Elisabeth
Chambers, Anna L
Koehler, Christine
Zou, Juan
Obong-Ebong, Ima
Kandiah, Eaazhisai
Temblador, Arturo
Round, Adam
Forest, Eric
Man, Petr
Bieniossek, Christoph
Laue, Ernest D
Lemke, Edward A
Rappsilber, Juri
Robinson, Carol V
Devys, Didier
Tora, Làszlò
Berger, Imre
author_facet Gupta, Kapil
Watson, Aleksandra A
Baptista, Tiago
Scheer, Elisabeth
Chambers, Anna L
Koehler, Christine
Zou, Juan
Obong-Ebong, Ima
Kandiah, Eaazhisai
Temblador, Arturo
Round, Adam
Forest, Eric
Man, Petr
Bieniossek, Christoph
Laue, Ernest D
Lemke, Edward A
Rappsilber, Juri
Robinson, Carol V
Devys, Didier
Tora, Làszlò
Berger, Imre
author_sort Gupta, Kapil
collection PubMed
description General transcription factor TFIID is a key component of RNA polymerase II transcription initiation. Human TFIID is a megadalton-sized complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core promoter DNA, recognizing the TATA-box. We identified a ternary complex formed by TBP and the histone fold (HF) domain-containing TFIID subunits TAF11 and TAF13. We demonstrate that TAF11/TAF13 competes for TBP binding with TATA-box DNA, and also with the N-terminal domain of TAF1 previously implicated in TATA-box mimicry. In an integrative approach combining crystal coordinates, biochemical analyses and data from cross-linking mass-spectrometry (CLMS), we determine the architecture of the TAF11/TAF13/TBP complex, revealing TAF11/TAF13 interaction with the DNA binding surface of TBP. We identify a highly conserved C-terminal TBP-interaction domain (CTID) in TAF13, which is essential for supporting cell growth. Our results thus have implications for cellular TFIID assembly and suggest a novel regulatory state for TFIID function.
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spelling pubmed-56902822017-11-20 Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID Gupta, Kapil Watson, Aleksandra A Baptista, Tiago Scheer, Elisabeth Chambers, Anna L Koehler, Christine Zou, Juan Obong-Ebong, Ima Kandiah, Eaazhisai Temblador, Arturo Round, Adam Forest, Eric Man, Petr Bieniossek, Christoph Laue, Ernest D Lemke, Edward A Rappsilber, Juri Robinson, Carol V Devys, Didier Tora, Làszlò Berger, Imre eLife Biochemistry and Chemical Biology General transcription factor TFIID is a key component of RNA polymerase II transcription initiation. Human TFIID is a megadalton-sized complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core promoter DNA, recognizing the TATA-box. We identified a ternary complex formed by TBP and the histone fold (HF) domain-containing TFIID subunits TAF11 and TAF13. We demonstrate that TAF11/TAF13 competes for TBP binding with TATA-box DNA, and also with the N-terminal domain of TAF1 previously implicated in TATA-box mimicry. In an integrative approach combining crystal coordinates, biochemical analyses and data from cross-linking mass-spectrometry (CLMS), we determine the architecture of the TAF11/TAF13/TBP complex, revealing TAF11/TAF13 interaction with the DNA binding surface of TBP. We identify a highly conserved C-terminal TBP-interaction domain (CTID) in TAF13, which is essential for supporting cell growth. Our results thus have implications for cellular TFIID assembly and suggest a novel regulatory state for TFIID function. eLife Sciences Publications, Ltd 2017-11-07 /pmc/articles/PMC5690282/ /pubmed/29111974 http://dx.doi.org/10.7554/eLife.30395 Text en © 2017, Gupta et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Gupta, Kapil
Watson, Aleksandra A
Baptista, Tiago
Scheer, Elisabeth
Chambers, Anna L
Koehler, Christine
Zou, Juan
Obong-Ebong, Ima
Kandiah, Eaazhisai
Temblador, Arturo
Round, Adam
Forest, Eric
Man, Petr
Bieniossek, Christoph
Laue, Ernest D
Lemke, Edward A
Rappsilber, Juri
Robinson, Carol V
Devys, Didier
Tora, Làszlò
Berger, Imre
Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
title Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
title_full Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
title_fullStr Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
title_full_unstemmed Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
title_short Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
title_sort architecture of taf11/taf13/tbp complex suggests novel regulation properties of general transcription factor tfiid
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690282/
https://www.ncbi.nlm.nih.gov/pubmed/29111974
http://dx.doi.org/10.7554/eLife.30395
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