Cargando…
High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls
Animal models suggest that chemokines are important mediators in the pathophysiology of neuropathic pain. Indeed, these substances have been called “gliotransmitters,” a term that illustrates the close interplay between glial cells and neurons in the context of neuroinflammation and pain. However, e...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690569/ https://www.ncbi.nlm.nih.gov/pubmed/28930774 http://dx.doi.org/10.1097/j.pain.0000000000001061 |
_version_ | 1783279629933477888 |
---|---|
author | Bäckryd, Emmanuel Lind, Anne-Li Thulin, Måns Larsson, Anders Gerdle, Björn Gordh, Torsten |
author_facet | Bäckryd, Emmanuel Lind, Anne-Li Thulin, Måns Larsson, Anders Gerdle, Björn Gordh, Torsten |
author_sort | Bäckryd, Emmanuel |
collection | PubMed |
description | Animal models suggest that chemokines are important mediators in the pathophysiology of neuropathic pain. Indeed, these substances have been called “gliotransmitters,” a term that illustrates the close interplay between glial cells and neurons in the context of neuroinflammation and pain. However, evidence in humans is scarce. The aim of the study was to determine a comprehensive cerebrospinal fluid (CSF) inflammatory profile of patients with neuropathic pain. Our hypothesis was that we would thereby find indications of a postulated on-going process of central neuroinflammation. Samples of CSF were collected from 2 cohorts of patients with neuropathic pain (n = 11 and n = 16, respectively) and healthy control subjects (n = 11). The samples were analyzed with a multiplex proximity extension assay in which 92 inflammation-related proteins were measured simultaneously (Proseek Multiplex Inflammation I; Olink Bioscience, Uppsala, Sweden). Univariate testing with control of false discovery rate, as well as orthogonal partial least squares discriminant analysis, were used for statistical analyses. Levels of chemokines CXCL6, CXCL10, CCL8, CCL11, CCL23 in CSF, as well as protein LAPTGF-beta-1, were significantly higher in both neuropathic pain cohorts compared with healthy controls, pointing to neuroinflammation in patients. These 6 proteins were also major results in a recent similar study in patients with fibromyalgia. The findings need to be confirmed in larger cohorts, and the question of causality remains to be settled. Because it has been suggested that prevalent comorbidities to chronic pain (eg, depression, anxiety, poor sleep, and tiredness) also are associated with neuroinflammation, it will be important to determine whether neuroinflammation is a common mediator. |
format | Online Article Text |
id | pubmed-5690569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Wolters Kluwer |
record_format | MEDLINE/PubMed |
spelling | pubmed-56905692017-11-29 High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls Bäckryd, Emmanuel Lind, Anne-Li Thulin, Måns Larsson, Anders Gerdle, Björn Gordh, Torsten Pain Research Paper Animal models suggest that chemokines are important mediators in the pathophysiology of neuropathic pain. Indeed, these substances have been called “gliotransmitters,” a term that illustrates the close interplay between glial cells and neurons in the context of neuroinflammation and pain. However, evidence in humans is scarce. The aim of the study was to determine a comprehensive cerebrospinal fluid (CSF) inflammatory profile of patients with neuropathic pain. Our hypothesis was that we would thereby find indications of a postulated on-going process of central neuroinflammation. Samples of CSF were collected from 2 cohorts of patients with neuropathic pain (n = 11 and n = 16, respectively) and healthy control subjects (n = 11). The samples were analyzed with a multiplex proximity extension assay in which 92 inflammation-related proteins were measured simultaneously (Proseek Multiplex Inflammation I; Olink Bioscience, Uppsala, Sweden). Univariate testing with control of false discovery rate, as well as orthogonal partial least squares discriminant analysis, were used for statistical analyses. Levels of chemokines CXCL6, CXCL10, CCL8, CCL11, CCL23 in CSF, as well as protein LAPTGF-beta-1, were significantly higher in both neuropathic pain cohorts compared with healthy controls, pointing to neuroinflammation in patients. These 6 proteins were also major results in a recent similar study in patients with fibromyalgia. The findings need to be confirmed in larger cohorts, and the question of causality remains to be settled. Because it has been suggested that prevalent comorbidities to chronic pain (eg, depression, anxiety, poor sleep, and tiredness) also are associated with neuroinflammation, it will be important to determine whether neuroinflammation is a common mediator. Wolters Kluwer 2017-09-18 2017-12 /pmc/articles/PMC5690569/ /pubmed/28930774 http://dx.doi.org/10.1097/j.pain.0000000000001061 Text en Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain. This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Bäckryd, Emmanuel Lind, Anne-Li Thulin, Måns Larsson, Anders Gerdle, Björn Gordh, Torsten High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls |
title | High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls |
title_full | High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls |
title_fullStr | High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls |
title_full_unstemmed | High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls |
title_short | High levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls |
title_sort | high levels of cerebrospinal fluid chemokines point to the presence of neuroinflammation in peripheral neuropathic pain: a cross-sectional study of 2 cohorts of patients compared with healthy controls |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690569/ https://www.ncbi.nlm.nih.gov/pubmed/28930774 http://dx.doi.org/10.1097/j.pain.0000000000001061 |
work_keys_str_mv | AT backrydemmanuel highlevelsofcerebrospinalfluidchemokinespointtothepresenceofneuroinflammationinperipheralneuropathicpainacrosssectionalstudyof2cohortsofpatientscomparedwithhealthycontrols AT lindanneli highlevelsofcerebrospinalfluidchemokinespointtothepresenceofneuroinflammationinperipheralneuropathicpainacrosssectionalstudyof2cohortsofpatientscomparedwithhealthycontrols AT thulinmans highlevelsofcerebrospinalfluidchemokinespointtothepresenceofneuroinflammationinperipheralneuropathicpainacrosssectionalstudyof2cohortsofpatientscomparedwithhealthycontrols AT larssonanders highlevelsofcerebrospinalfluidchemokinespointtothepresenceofneuroinflammationinperipheralneuropathicpainacrosssectionalstudyof2cohortsofpatientscomparedwithhealthycontrols AT gerdlebjorn highlevelsofcerebrospinalfluidchemokinespointtothepresenceofneuroinflammationinperipheralneuropathicpainacrosssectionalstudyof2cohortsofpatientscomparedwithhealthycontrols AT gordhtorsten highlevelsofcerebrospinalfluidchemokinespointtothepresenceofneuroinflammationinperipheralneuropathicpainacrosssectionalstudyof2cohortsofpatientscomparedwithhealthycontrols |