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The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes

B-1 lymphocytes are known to increase the metastatic potential of B16F10 melanoma cells via the extracellular signal-regulated kinase (ERK) pathway. Since IL-10 is associated with B-1 cells performance, we hypothesized that IL-10 could be implicated in the progression of melanoma. In the present wor...

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Autores principales: Perez, Elizabeth Cristina, Xander, Patricia, Laurindo, Maria Fernanda Lucatelli, Novaes e Brito, Ronni Rômulo, Vivanco, Bruno Camolese, Mortara, Renato Arruda, Mariano, Mario, Lopes, José Daniel, Keller, Alexandre Castro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690663/
https://www.ncbi.nlm.nih.gov/pubmed/29145406
http://dx.doi.org/10.1371/journal.pone.0187333
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author Perez, Elizabeth Cristina
Xander, Patricia
Laurindo, Maria Fernanda Lucatelli
Novaes e Brito, Ronni Rômulo
Vivanco, Bruno Camolese
Mortara, Renato Arruda
Mariano, Mario
Lopes, José Daniel
Keller, Alexandre Castro
author_facet Perez, Elizabeth Cristina
Xander, Patricia
Laurindo, Maria Fernanda Lucatelli
Novaes e Brito, Ronni Rômulo
Vivanco, Bruno Camolese
Mortara, Renato Arruda
Mariano, Mario
Lopes, José Daniel
Keller, Alexandre Castro
author_sort Perez, Elizabeth Cristina
collection PubMed
description B-1 lymphocytes are known to increase the metastatic potential of B16F10 melanoma cells via the extracellular signal-regulated kinase (ERK) pathway. Since IL-10 is associated with B-1 cells performance, we hypothesized that IL-10 could be implicated in the progression of melanoma. In the present work, we found that the C57BL/6 mice, inoculated with B16F10 cells that were co-cultivated with B-1 lymphocytes from IL-10 knockout mice, developed fewer metastatic nodules than the ones which were injected with the melanoma cells that were cultivated in the presence of wild-type B-1 cells. The impairment of metastatic potential of the B16F10 cells was correlated with low activation of the ERK signaling pathway, supporting the idea that the production of IL-10 by B-1 cells influences the behavior of the tumor. A microarray analysis of the B-1 lymphocytes revealed that IL-10 deficiency is associated with down-regulation of the genes that code for claudin-10, a protein that is involved in cell-to-cell contact and that has been linked to lung adenocarcinoma. In order to determine the impact of claudin-10 in the cross-talk between B-1 lymphocytes and the B16F10 tumor cells, we took advantage of small interfering RNA. The silencing of claudin-10 gene in B-1 lymphocytes inhibited activation of the ERK pathway and abrogated the B-1-induced aggressive behavior of the B16F10 cells. Thus, our findings suggest that the axis IL-10/claudin-10 is a promising target for the development of therapeutic agents against aggressive melanoma.
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spelling pubmed-56906632017-11-30 The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes Perez, Elizabeth Cristina Xander, Patricia Laurindo, Maria Fernanda Lucatelli Novaes e Brito, Ronni Rômulo Vivanco, Bruno Camolese Mortara, Renato Arruda Mariano, Mario Lopes, José Daniel Keller, Alexandre Castro PLoS One Research Article B-1 lymphocytes are known to increase the metastatic potential of B16F10 melanoma cells via the extracellular signal-regulated kinase (ERK) pathway. Since IL-10 is associated with B-1 cells performance, we hypothesized that IL-10 could be implicated in the progression of melanoma. In the present work, we found that the C57BL/6 mice, inoculated with B16F10 cells that were co-cultivated with B-1 lymphocytes from IL-10 knockout mice, developed fewer metastatic nodules than the ones which were injected with the melanoma cells that were cultivated in the presence of wild-type B-1 cells. The impairment of metastatic potential of the B16F10 cells was correlated with low activation of the ERK signaling pathway, supporting the idea that the production of IL-10 by B-1 cells influences the behavior of the tumor. A microarray analysis of the B-1 lymphocytes revealed that IL-10 deficiency is associated with down-regulation of the genes that code for claudin-10, a protein that is involved in cell-to-cell contact and that has been linked to lung adenocarcinoma. In order to determine the impact of claudin-10 in the cross-talk between B-1 lymphocytes and the B16F10 tumor cells, we took advantage of small interfering RNA. The silencing of claudin-10 gene in B-1 lymphocytes inhibited activation of the ERK pathway and abrogated the B-1-induced aggressive behavior of the B16F10 cells. Thus, our findings suggest that the axis IL-10/claudin-10 is a promising target for the development of therapeutic agents against aggressive melanoma. Public Library of Science 2017-11-16 /pmc/articles/PMC5690663/ /pubmed/29145406 http://dx.doi.org/10.1371/journal.pone.0187333 Text en © 2017 Perez et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Perez, Elizabeth Cristina
Xander, Patricia
Laurindo, Maria Fernanda Lucatelli
Novaes e Brito, Ronni Rômulo
Vivanco, Bruno Camolese
Mortara, Renato Arruda
Mariano, Mario
Lopes, José Daniel
Keller, Alexandre Castro
The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes
title The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes
title_full The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes
title_fullStr The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes
title_full_unstemmed The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes
title_short The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes
title_sort axis il-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by b-1 lymphocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690663/
https://www.ncbi.nlm.nih.gov/pubmed/29145406
http://dx.doi.org/10.1371/journal.pone.0187333
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