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Engineering the GH1 β-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose

The activity of the GH1 β-glucosidase from Humicola insolens (Bglhi) against p-nitrophenyl-β-D-glucopyranoside (pNP-Glc) and cellobiose is enhanced 2-fold by glucose and/or xylose. Kinetic and transglycosylation data showed that hydrolysis is preferred in the absence of monosaccharides. Stimulation...

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Detalles Bibliográficos
Autores principales:	Meleiro, Luana Parras, Salgado, José Carlos Santos, Maldonado, Raquel Fonseca, Carli, Sibeli, Moraes, Luiz Alberto Beraldo, Ward, Richard John, Jorge, João Atílio, Furriel, Rosa Prazeres Melo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5690678/ https://www.ncbi.nlm.nih.gov/pubmed/29145480 http://dx.doi.org/10.1371/journal.pone.0188254

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