Variations in nutrient and trace element composition of rice in an organic rice-frog coculture system

Introducing frogs into paddy fields can control pests and diseases, and organic farming can improve soil fertility and rice growth. The aim of this 2-year field study was compare the yield and elemental composition of rice between an organic farming system including frogs (ORF) and a conventional rice culture system (CR). The grain yields were almost the same in the ORF system and the CR system. The ORF significantly increased the contents of phosphorus (P), ion (Fe), zinc (Zn), molybdenum (Mo) and selenium (Se) in rice grain at one or both years. However, the ORF system decreased the calcium (Ca) content in grice grains, and increased the concentration of cadmium, which is potentially toxic. A principal components analysis showed the main impacts of ORF agro-ecosystem on the rice grain ionome was to increase the concentration of P and trace metal(loid)s. The results showed that the ORF system is an ecologically, friendly strategy to avoid excessive use of chemical fertilizers, herbicides and pesticides without decreasing yields, and to improve the nutritional status of rice by increasing the micronutrient contents. The potential risks of increasing Cd contents in rice grain should be addressed if this cultivation pattern is used in the long term.