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Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates

Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster fro...

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Autores principales: Yablonovitch, Arielle L., Fu, Jeremy, Li, Kexin, Mahato, Simpla, Kang, Lin, Rashkovetsky, Eugenia, Korol, Abraham B., Tang, Hua, Michalak, Pawel, Zelhof, Andrew C., Nevo, Eviatar, Li, Jin Billy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691062/
https://www.ncbi.nlm.nih.gov/pubmed/29146998
http://dx.doi.org/10.1038/s41467-017-01658-2
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author Yablonovitch, Arielle L.
Fu, Jeremy
Li, Kexin
Mahato, Simpla
Kang, Lin
Rashkovetsky, Eugenia
Korol, Abraham B.
Tang, Hua
Michalak, Pawel
Zelhof, Andrew C.
Nevo, Eviatar
Li, Jin Billy
author_facet Yablonovitch, Arielle L.
Fu, Jeremy
Li, Kexin
Mahato, Simpla
Kang, Lin
Rashkovetsky, Eugenia
Korol, Abraham B.
Tang, Hua
Michalak, Pawel
Zelhof, Andrew C.
Nevo, Eviatar
Li, Jin Billy
author_sort Yablonovitch, Arielle L.
collection PubMed
description Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from ‘Evolution Canyon’ in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, genetically regulated sites tend to be less affected by temperature. This work shows the extent to which gene expression and RNA editing differ between flies from different microclimates, and provides insights into the regulation responsible for these differences.
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spelling pubmed-56910622017-11-20 Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates Yablonovitch, Arielle L. Fu, Jeremy Li, Kexin Mahato, Simpla Kang, Lin Rashkovetsky, Eugenia Korol, Abraham B. Tang, Hua Michalak, Pawel Zelhof, Andrew C. Nevo, Eviatar Li, Jin Billy Nat Commun Article Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from ‘Evolution Canyon’ in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, genetically regulated sites tend to be less affected by temperature. This work shows the extent to which gene expression and RNA editing differ between flies from different microclimates, and provides insights into the regulation responsible for these differences. Nature Publishing Group UK 2017-11-17 /pmc/articles/PMC5691062/ /pubmed/29146998 http://dx.doi.org/10.1038/s41467-017-01658-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yablonovitch, Arielle L.
Fu, Jeremy
Li, Kexin
Mahato, Simpla
Kang, Lin
Rashkovetsky, Eugenia
Korol, Abraham B.
Tang, Hua
Michalak, Pawel
Zelhof, Andrew C.
Nevo, Eviatar
Li, Jin Billy
Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates
title Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates
title_full Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates
title_fullStr Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates
title_full_unstemmed Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates
title_short Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates
title_sort regulation of gene expression and rna editing in drosophila adapting to divergent microclimates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691062/
https://www.ncbi.nlm.nih.gov/pubmed/29146998
http://dx.doi.org/10.1038/s41467-017-01658-2
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