How gluttonous cell aggregates clear substrates coated with microparticles

We study the spreading of cell aggregates deposited on adhesive substrates decorated with microparticles (MPs). A cell monolayer expands around the aggregate. The cells on the periphery of the monolayer take up the MPs, clearing the substrate as they progress and forming an aureole of cells filled w...

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Autores principales: Beaune, Grégory, Lam, Andy Y. W., Dufour, Sylvie, Winnik, Françoise M., Brochard-Wyart, Françoise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691206/
https://www.ncbi.nlm.nih.gov/pubmed/29146990
http://dx.doi.org/10.1038/s41598-017-15665-2
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author Beaune, Grégory
Lam, Andy Y. W.
Dufour, Sylvie
Winnik, Françoise M.
Brochard-Wyart, Françoise
author_facet Beaune, Grégory
Lam, Andy Y. W.
Dufour, Sylvie
Winnik, Françoise M.
Brochard-Wyart, Françoise
author_sort Beaune, Grégory
collection PubMed
description We study the spreading of cell aggregates deposited on adhesive substrates decorated with microparticles (MPs). A cell monolayer expands around the aggregate. The cells on the periphery of the monolayer take up the MPs, clearing the substrate as they progress and forming an aureole of cells filled with MPs. We study the dynamics of spreading and determine the width of the aureole and the level of MP internalization in cells as a function of MP size, composition, and density. From the radius and width of the aureole, we quantify the volume fraction of MPs within the cell, which leads to an easy, fast, and inexpensive measurement of the cell – particle internalization.
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spelling pubmed-56912062017-11-24 How gluttonous cell aggregates clear substrates coated with microparticles Beaune, Grégory Lam, Andy Y. W. Dufour, Sylvie Winnik, Françoise M. Brochard-Wyart, Françoise Sci Rep Article We study the spreading of cell aggregates deposited on adhesive substrates decorated with microparticles (MPs). A cell monolayer expands around the aggregate. The cells on the periphery of the monolayer take up the MPs, clearing the substrate as they progress and forming an aureole of cells filled with MPs. We study the dynamics of spreading and determine the width of the aureole and the level of MP internalization in cells as a function of MP size, composition, and density. From the radius and width of the aureole, we quantify the volume fraction of MPs within the cell, which leads to an easy, fast, and inexpensive measurement of the cell – particle internalization. Nature Publishing Group UK 2017-11-16 /pmc/articles/PMC5691206/ /pubmed/29146990 http://dx.doi.org/10.1038/s41598-017-15665-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Beaune, Grégory
Lam, Andy Y. W.
Dufour, Sylvie
Winnik, Françoise M.
Brochard-Wyart, Françoise
How gluttonous cell aggregates clear substrates coated with microparticles
title How gluttonous cell aggregates clear substrates coated with microparticles
title_full How gluttonous cell aggregates clear substrates coated with microparticles
title_fullStr How gluttonous cell aggregates clear substrates coated with microparticles
title_full_unstemmed How gluttonous cell aggregates clear substrates coated with microparticles
title_short How gluttonous cell aggregates clear substrates coated with microparticles
title_sort how gluttonous cell aggregates clear substrates coated with microparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691206/
https://www.ncbi.nlm.nih.gov/pubmed/29146990
http://dx.doi.org/10.1038/s41598-017-15665-2
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