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Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice

INTRODUCTION: Organ‐specific autoimmune diseases are believed to result from immune responses generated against self‐antigens specific to each organ. However, when such responses target antigens expressed promiscuously in multiple tissues, then the immune‐mediated damage may be wide spread. METHODS:...

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Autores principales: Krishnan, Bharathi, Massilamany, Chandirasegaran, Basavalingappa, Rakesh H., Gangaplara, Arunakumar, Kang, Guobin, Li, Qingsheng, Uzal, Francisco A., Strande, Jennifer L., Delhon, Gustavo A., Riethoven, Jean‐Jack, Steffen, David, Reddy, Jay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691315/
https://www.ncbi.nlm.nih.gov/pubmed/28597552
http://dx.doi.org/10.1002/iid3.177
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author Krishnan, Bharathi
Massilamany, Chandirasegaran
Basavalingappa, Rakesh H.
Gangaplara, Arunakumar
Kang, Guobin
Li, Qingsheng
Uzal, Francisco A.
Strande, Jennifer L.
Delhon, Gustavo A.
Riethoven, Jean‐Jack
Steffen, David
Reddy, Jay
author_facet Krishnan, Bharathi
Massilamany, Chandirasegaran
Basavalingappa, Rakesh H.
Gangaplara, Arunakumar
Kang, Guobin
Li, Qingsheng
Uzal, Francisco A.
Strande, Jennifer L.
Delhon, Gustavo A.
Riethoven, Jean‐Jack
Steffen, David
Reddy, Jay
author_sort Krishnan, Bharathi
collection PubMed
description INTRODUCTION: Organ‐specific autoimmune diseases are believed to result from immune responses generated against self‐antigens specific to each organ. However, when such responses target antigens expressed promiscuously in multiple tissues, then the immune‐mediated damage may be wide spread. METHODS: In this report, we describe a mitochondrial protein, branched chain α‐ketoacid dehydrogenase kinase (BCKD(k)) that can act as a target autoantigen in the development of autoimmune inflammatory reactions in both heart and liver. RESULTS: We demonstrate that BCKD(k) protein contains at least nine immunodominant epitopes, three of which, BCKD(k) 71–90, BCKD(k) 111–130 and BCKD(k) 141–160, were found to induce varying degrees of myocarditis in immunized mice. One of these, BCKD(k) 111–130, could also induce hepatitis without affecting lungs, kidneys, skeletal muscles, and brain. In immunogenicity testing, all three peptides induced antigen‐specific T cell responses, as verified by proliferation assay and/or major histocompatibility complex class II/IA(k) dextramer staining. Finally, the disease‐inducing abilities of BCKD(k) peptides were correlated with the production of interferon‐γ, and the activated T cells could transfer disease to naive recipients. CONCLUSIONS: The disease induced by BCKD(k) peptides could serve as a useful model to study the autoimmune events of inflammatory heart and liver diseases.
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spelling pubmed-56913152017-11-24 Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice Krishnan, Bharathi Massilamany, Chandirasegaran Basavalingappa, Rakesh H. Gangaplara, Arunakumar Kang, Guobin Li, Qingsheng Uzal, Francisco A. Strande, Jennifer L. Delhon, Gustavo A. Riethoven, Jean‐Jack Steffen, David Reddy, Jay Immun Inflamm Dis Original Research INTRODUCTION: Organ‐specific autoimmune diseases are believed to result from immune responses generated against self‐antigens specific to each organ. However, when such responses target antigens expressed promiscuously in multiple tissues, then the immune‐mediated damage may be wide spread. METHODS: In this report, we describe a mitochondrial protein, branched chain α‐ketoacid dehydrogenase kinase (BCKD(k)) that can act as a target autoantigen in the development of autoimmune inflammatory reactions in both heart and liver. RESULTS: We demonstrate that BCKD(k) protein contains at least nine immunodominant epitopes, three of which, BCKD(k) 71–90, BCKD(k) 111–130 and BCKD(k) 141–160, were found to induce varying degrees of myocarditis in immunized mice. One of these, BCKD(k) 111–130, could also induce hepatitis without affecting lungs, kidneys, skeletal muscles, and brain. In immunogenicity testing, all three peptides induced antigen‐specific T cell responses, as verified by proliferation assay and/or major histocompatibility complex class II/IA(k) dextramer staining. Finally, the disease‐inducing abilities of BCKD(k) peptides were correlated with the production of interferon‐γ, and the activated T cells could transfer disease to naive recipients. CONCLUSIONS: The disease induced by BCKD(k) peptides could serve as a useful model to study the autoimmune events of inflammatory heart and liver diseases. John Wiley and Sons Inc. 2017-06-09 /pmc/articles/PMC5691315/ /pubmed/28597552 http://dx.doi.org/10.1002/iid3.177 Text en © 2017 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Krishnan, Bharathi
Massilamany, Chandirasegaran
Basavalingappa, Rakesh H.
Gangaplara, Arunakumar
Kang, Guobin
Li, Qingsheng
Uzal, Francisco A.
Strande, Jennifer L.
Delhon, Gustavo A.
Riethoven, Jean‐Jack
Steffen, David
Reddy, Jay
Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice
title Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice
title_full Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice
title_fullStr Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice
title_full_unstemmed Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice
title_short Branched chain α‐ketoacid dehydrogenase kinase 111–130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice
title_sort branched chain α‐ketoacid dehydrogenase kinase 111–130, a t cell epitope that induces both autoimmune myocarditis and hepatitis in a/j mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691315/
https://www.ncbi.nlm.nih.gov/pubmed/28597552
http://dx.doi.org/10.1002/iid3.177
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