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The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid

Trimethylamine-N-oxide (TMAO) is a small organic molecule, derived from the intestinal and hepatic metabolism of dietary choline and carnitine. Although the involvement of TMAO in the framework of many chronic diseases has been recently described, no evidence on its putative role in the central nerv...

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Autores principales: Rio, Daniele Del, Zimetti, Francesca, Caffarra, Paolo, Tassotti, Michele, Bernini, Franco, Brighenti, Furio, Zini, Andrea, Zanotti, Ilaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691670/
https://www.ncbi.nlm.nih.gov/pubmed/28937600
http://dx.doi.org/10.3390/nu9101053
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author Rio, Daniele Del
Zimetti, Francesca
Caffarra, Paolo
Tassotti, Michele
Bernini, Franco
Brighenti, Furio
Zini, Andrea
Zanotti, Ilaria
author_facet Rio, Daniele Del
Zimetti, Francesca
Caffarra, Paolo
Tassotti, Michele
Bernini, Franco
Brighenti, Furio
Zini, Andrea
Zanotti, Ilaria
author_sort Rio, Daniele Del
collection PubMed
description Trimethylamine-N-oxide (TMAO) is a small organic molecule, derived from the intestinal and hepatic metabolism of dietary choline and carnitine. Although the involvement of TMAO in the framework of many chronic diseases has been recently described, no evidence on its putative role in the central nervous system has been provided. The aim of this study was to evaluate whether TMAO is present at detectable levels in human cerebrospinal fluid (CSF). CSF was collected for diagnostic purposes from 58 subjects by lumbar puncture and TMAO was quantified by using liquid chromatography coupled with multiple-reaction monitoring mass spectrometry. The molecule was detected in all samples, at concentrations ranging between 0.11 and 6.43 µmol/L. Further analysis on CSF revealed that a total of 22 subjects were affected by Alzheimer’s disease (AD), 16 were affected by non-AD related dementia, and 20 were affected by other neurological disorders. However, the stratification of TMAO levels according to the neurological diagnoses revealed no differences among the three groups. In conclusion, we provide the first evidence that TMAO can be assessed in human CSF, but the actual impact of this dietary metabolite in the patho-physiolgy of the central nervous system requires further study.
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spelling pubmed-56916702017-11-22 The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid Rio, Daniele Del Zimetti, Francesca Caffarra, Paolo Tassotti, Michele Bernini, Franco Brighenti, Furio Zini, Andrea Zanotti, Ilaria Nutrients Communication Trimethylamine-N-oxide (TMAO) is a small organic molecule, derived from the intestinal and hepatic metabolism of dietary choline and carnitine. Although the involvement of TMAO in the framework of many chronic diseases has been recently described, no evidence on its putative role in the central nervous system has been provided. The aim of this study was to evaluate whether TMAO is present at detectable levels in human cerebrospinal fluid (CSF). CSF was collected for diagnostic purposes from 58 subjects by lumbar puncture and TMAO was quantified by using liquid chromatography coupled with multiple-reaction monitoring mass spectrometry. The molecule was detected in all samples, at concentrations ranging between 0.11 and 6.43 µmol/L. Further analysis on CSF revealed that a total of 22 subjects were affected by Alzheimer’s disease (AD), 16 were affected by non-AD related dementia, and 20 were affected by other neurological disorders. However, the stratification of TMAO levels according to the neurological diagnoses revealed no differences among the three groups. In conclusion, we provide the first evidence that TMAO can be assessed in human CSF, but the actual impact of this dietary metabolite in the patho-physiolgy of the central nervous system requires further study. MDPI 2017-09-22 /pmc/articles/PMC5691670/ /pubmed/28937600 http://dx.doi.org/10.3390/nu9101053 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Rio, Daniele Del
Zimetti, Francesca
Caffarra, Paolo
Tassotti, Michele
Bernini, Franco
Brighenti, Furio
Zini, Andrea
Zanotti, Ilaria
The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid
title The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid
title_full The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid
title_fullStr The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid
title_full_unstemmed The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid
title_short The Gut Microbial Metabolite Trimethylamine-N-Oxide Is Present in Human Cerebrospinal Fluid
title_sort gut microbial metabolite trimethylamine-n-oxide is present in human cerebrospinal fluid
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691670/
https://www.ncbi.nlm.nih.gov/pubmed/28937600
http://dx.doi.org/10.3390/nu9101053
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