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Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group
The Puccinia graminis f. sp. tritici (Pgt) Ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. The durum wheat (Triticum turgidum ssp. durum) gene Sr13 confers resistance to Ug99 and other virulent races, and is mo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5692537/ https://www.ncbi.nlm.nih.gov/pubmed/29078294 http://dx.doi.org/10.1073/pnas.1706277114 |
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author | Zhang, Wenjun Chen, Shisheng Abate, Zewdie Nirmala, Jayaveeramuthu Rouse, Matthew N. Dubcovsky, Jorge |
author_facet | Zhang, Wenjun Chen, Shisheng Abate, Zewdie Nirmala, Jayaveeramuthu Rouse, Matthew N. Dubcovsky, Jorge |
author_sort | Zhang, Wenjun |
collection | PubMed |
description | The Puccinia graminis f. sp. tritici (Pgt) Ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. The durum wheat (Triticum turgidum ssp. durum) gene Sr13 confers resistance to Ug99 and other virulent races, and is more effective at high temperatures. Using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated CNL3 and CNL13. Three independent truncation mutations identified in each of these genes demonstrated that only CNL13 was required for Ug99 resistance. Transformation of an 8-kb genomic sequence including CNL13 into the susceptible wheat variety Fielder was sufficient to confer resistance to Ug99, confirming that CNL13 is Sr13. CNL13 transcripts were slightly down-regulated 2–6 days after Pgt inoculation and were not affected by temperature. By contrast, six pathogenesis-related (PR) genes were up-regulated at high temperatures only when both Sr13 and Pgt were present, suggesting that they may contribute to the high temperature resistance mechanism. We identified three Sr13-resistant haplotypes, which were present in one-third of cultivated emmer and durum wheats but absent in most tested common wheats (Triticum aestivum). These results suggest that Sr13 can be used to improve Ug99 resistance in a large proportion of modern wheat cultivars. To accelerate its deployment, we developed a diagnostic marker for Sr13. The identification of Sr13 expands the number of Pgt-resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease. |
format | Online Article Text |
id | pubmed-5692537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-56925372017-11-20 Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group Zhang, Wenjun Chen, Shisheng Abate, Zewdie Nirmala, Jayaveeramuthu Rouse, Matthew N. Dubcovsky, Jorge Proc Natl Acad Sci U S A PNAS Plus The Puccinia graminis f. sp. tritici (Pgt) Ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. The durum wheat (Triticum turgidum ssp. durum) gene Sr13 confers resistance to Ug99 and other virulent races, and is more effective at high temperatures. Using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated CNL3 and CNL13. Three independent truncation mutations identified in each of these genes demonstrated that only CNL13 was required for Ug99 resistance. Transformation of an 8-kb genomic sequence including CNL13 into the susceptible wheat variety Fielder was sufficient to confer resistance to Ug99, confirming that CNL13 is Sr13. CNL13 transcripts were slightly down-regulated 2–6 days after Pgt inoculation and were not affected by temperature. By contrast, six pathogenesis-related (PR) genes were up-regulated at high temperatures only when both Sr13 and Pgt were present, suggesting that they may contribute to the high temperature resistance mechanism. We identified three Sr13-resistant haplotypes, which were present in one-third of cultivated emmer and durum wheats but absent in most tested common wheats (Triticum aestivum). These results suggest that Sr13 can be used to improve Ug99 resistance in a large proportion of modern wheat cultivars. To accelerate its deployment, we developed a diagnostic marker for Sr13. The identification of Sr13 expands the number of Pgt-resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease. National Academy of Sciences 2017-11-07 2017-10-23 /pmc/articles/PMC5692537/ /pubmed/29078294 http://dx.doi.org/10.1073/pnas.1706277114 Text en Copyright © 2017 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .https://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | PNAS Plus Zhang, Wenjun Chen, Shisheng Abate, Zewdie Nirmala, Jayaveeramuthu Rouse, Matthew N. Dubcovsky, Jorge Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group |
title | Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group |
title_full | Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group |
title_fullStr | Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group |
title_full_unstemmed | Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group |
title_short | Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group |
title_sort | identification and characterization of sr13, a tetraploid wheat gene that confers resistance to the ug99 stem rust race group |
topic | PNAS Plus |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5692537/ https://www.ncbi.nlm.nih.gov/pubmed/29078294 http://dx.doi.org/10.1073/pnas.1706277114 |
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