cGAL, a Temperature-Robust GAL4-UAS System for C. elegans

The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in C. elegans has not been described. Here we systematically optimize the system’s three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C....

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Detalles Bibliográficos
Autores principales: Wang, Han, Liu, Jonathan, Gharib, Shahla, Chai, Cynthia M., Schwarz, Erich M., Pokala, Navin, Sternberg, Paul W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693259/
https://www.ncbi.nlm.nih.gov/pubmed/27992408
http://dx.doi.org/10.1038/nmeth.4109
Descripción
Sumario:The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in C. elegans has not been described. Here we systematically optimize the system’s three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans across 15–25°C. We demonstrate its utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression, and provide a basic driver and effector toolkit.

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