cGAL, a Temperature-Robust GAL4-UAS System for C. elegans

The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in C. elegans has not been described. Here we systematically optimize the system’s three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C....

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Autores principales: Wang, Han, Liu, Jonathan, Gharib, Shahla, Chai, Cynthia M., Schwarz, Erich M., Pokala, Navin, Sternberg, Paul W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693259/
https://www.ncbi.nlm.nih.gov/pubmed/27992408
http://dx.doi.org/10.1038/nmeth.4109
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author Wang, Han
Liu, Jonathan
Gharib, Shahla
Chai, Cynthia M.
Schwarz, Erich M.
Pokala, Navin
Sternberg, Paul W.
author_facet Wang, Han
Liu, Jonathan
Gharib, Shahla
Chai, Cynthia M.
Schwarz, Erich M.
Pokala, Navin
Sternberg, Paul W.
author_sort Wang, Han
collection PubMed
description The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in C. elegans has not been described. Here we systematically optimize the system’s three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans across 15–25°C. We demonstrate its utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression, and provide a basic driver and effector toolkit.
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spelling pubmed-56932592017-11-17 cGAL, a Temperature-Robust GAL4-UAS System for C. elegans Wang, Han Liu, Jonathan Gharib, Shahla Chai, Cynthia M. Schwarz, Erich M. Pokala, Navin Sternberg, Paul W. Nat Methods Article The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in C. elegans has not been described. Here we systematically optimize the system’s three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans across 15–25°C. We demonstrate its utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression, and provide a basic driver and effector toolkit. 2016-12-19 2017-02 /pmc/articles/PMC5693259/ /pubmed/27992408 http://dx.doi.org/10.1038/nmeth.4109 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Han
Liu, Jonathan
Gharib, Shahla
Chai, Cynthia M.
Schwarz, Erich M.
Pokala, Navin
Sternberg, Paul W.
cGAL, a Temperature-Robust GAL4-UAS System for C. elegans
title cGAL, a Temperature-Robust GAL4-UAS System for C. elegans
title_full cGAL, a Temperature-Robust GAL4-UAS System for C. elegans
title_fullStr cGAL, a Temperature-Robust GAL4-UAS System for C. elegans
title_full_unstemmed cGAL, a Temperature-Robust GAL4-UAS System for C. elegans
title_short cGAL, a Temperature-Robust GAL4-UAS System for C. elegans
title_sort cgal, a temperature-robust gal4-uas system for c. elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693259/
https://www.ncbi.nlm.nih.gov/pubmed/27992408
http://dx.doi.org/10.1038/nmeth.4109
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