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cGAL, a Temperature-Robust GAL4-UAS System for C. elegans
The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in C. elegans has not been described. Here we systematically optimize the system’s three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C....
Autores principales: | Wang, Han, Liu, Jonathan, Gharib, Shahla, Chai, Cynthia M., Schwarz, Erich M., Pokala, Navin, Sternberg, Paul W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5693259/ https://www.ncbi.nlm.nih.gov/pubmed/27992408 http://dx.doi.org/10.1038/nmeth.4109 |
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